Klymkowsky Michael W
Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado 80309
Cold Spring Harb Protoc. 2018 Jan 2;2018(1):pdb.prot097295. doi: 10.1101/pdb.prot097295.
To visualize the effects of experimental perturbations on normal cellular behavior, morphology, and intracellular organization, we use a simple whole-mount immunocytochemical method with oocytes, explants, or embryos. This method is applicable to a wide range of systems, including human-induced pluripotent stem cell-derived organoids, and can be used with both chromogenic (horseradish peroxidase/diaminobenzidine) and fluorescent imaging.
为了观察实验性扰动对正常细胞行为、形态和细胞内组织的影响，我们对卵母细胞、外植体或胚胎使用了一种简单的整装免疫细胞化学方法。该方法适用于广泛的系统，包括人诱导多能干细胞衍生的类器官，并且可用于显色成像（辣根过氧化物酶/二氨基联苯胺）和荧光成像。
