桦木酸诱导MDA-MB-231乳腺癌细胞凋亡及超微结构变化。

Betulinic acid induces apoptosis and ultrastructural changes in MDA-MB-231 breast cancer cells.

作者信息

Gao Yang, Ma Qing, Ma Yan-Bin, Ding Liang, Xu Xiao-Long, Wei De-Fei, Wei Lei, Zhang Jing-Wei

机构信息

a Department of Breast and Thyroid Surgery , Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan University , Wuhan , Hubei , China.

b Department of Pathology and Pathophysiology , Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University , Wuhan , Hubei , China.

出版信息

Ultrastruct Pathol. 2018 Jan-Feb;42(1):49-54. doi: 10.1080/01913123.2017.1383548. Epub 2017 Dec 1.

DOI:10.1080/01913123.2017.1383548

PMID:29192840

Abstract

The aim of this study is to investigate the effects of betulinic acid (BA) on triple-negative breast cancer MDA-MB-231 cells and observe the ultrastructural changes. The concentration of BA required to induce apoptosis in MDA-MB-231 cells has been previously reported. In this study, a cell counting kit-8 proliferation assay was used to measure cell viability and the apoptosis rate. Western blotting was performed to observe the protein expression levels of Bcl-2. Cell morphology and changes in cell density were observed by microscopy. Electron microscopy revealed pyknotic nuclei as well as vacuoles. Collectively, our results showed the morphological mechanisms by which BA impairs the ultrastructure of MDA-MB-231 cells.

摘要

本研究旨在探讨桦木酸（BA）对三阴性乳腺癌MDA-MB-231细胞的影响，并观察其超微结构变化。此前已有报道诱导MDA-MB-231细胞凋亡所需的BA浓度。在本研究中，使用细胞计数试剂盒-8增殖测定法来测量细胞活力和凋亡率。进行蛋白质免疫印迹法以观察Bcl-2的蛋白质表达水平。通过显微镜观察细胞形态和细胞密度变化。电子显微镜显示细胞核固缩以及空泡。总体而言，我们的结果显示了BA损害MDA-MB-231细胞超微结构的形态学机制。