Department of Dermatology, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.
Department of Dermatology & Venereology, Peking University First Hospital and Research Center for Medical Mycology, Peking University, Beijing, China.
Mycoses. 2018 Apr;61(4):279-282. doi: 10.1111/myc.12733. Epub 2017 Dec 26.
Onychomycosis is a common fungal infection in dermatology clinics. The commonly used diagnostic method, potassium hydroxide (KOH) direct microscopy, does not have a high-positive detection rate. Therefore, a new diagnostic method is needed to confirm onychomycosis. The objective of this research was to use fluorescent brightener 85 (FB 85), a new fluorescent reagent for fungi detection, and to evaluate this new method based on the diagnostic positive rate, accuracy and efficiency in suspected onychomycosis compared with the KOH method. Subungual debris was collected from clinically suspected onychomycosis cases and divided into two parts. The parts were examined by FB 85 and 10% KOH solution, respectively. In total, 108 patients with suspected onychomycosis were recruited. The positive rates of FB 85 and KOH were 88.9% and 55.6%, respectively. The patients showed significant statistical differences (P < .001). Compared with the KOH method, the FB 85 fluorescent method was an accurate and efficient method with a higher positive rate. This method could increase the diagnostic accuracy and efficiency of onychomycosis detection.
甲真菌病是皮肤科常见的真菌感染。常用的诊断方法氢氧化钾（KOH）直接镜检阳性率不高。因此，需要一种新的诊断方法来确诊甲真菌病。本研究旨在使用新型真菌荧光增白剂 85（FB 85），并基于诊断阳性率、准确性和效率，与 KOH 方法比较，评估该方法在疑似甲真菌病中的应用。从临床疑似甲真菌病患者中采集甲下碎屑并分为两部分，分别用 FB 85 和 10% KOH 溶液进行检查。共招募 108 例疑似甲真菌病患者，FB 85 和 KOH 的阳性率分别为 88.9%和 55.6%，差异有统计学意义（P<.001）。与 KOH 方法相比，FB 85 荧光法是一种准确、高效的方法，阳性率更高。该方法可提高甲真菌病检测的诊断准确性和效率。
