Abdul Manan Fatin Myra, Attan Nursyafreena, Widodo Nashi, Aboul-Enein Hassan Y, Wahab Roswanira Abdul
a Department of Chemistry, Faculty of Science , Universiti Teknologi Malaysia , Skudai , Malaysia.
b Faculty of Mathematics and Natural Sciences , Universitas Brawijaya , Malang , Indonesia.
Prep Biochem Biotechnol. 2018 Jan 2;48(1):92-102. doi: 10.1080/10826068.2017.1405021. Epub 2018 Jan 3.
An alternative environmentally benign support was prepared from chitosan-chitin nanowhiskers (CS/CNWs) for covalent immobilization of Rhizomucor miehei lipase (RML) to increase the operational stability and recyclability of RML in synthesizing eugenyl benzoate. The CS/CNWs support and RML-CS/CNWs were characterized using X-ray diffraction, fluorescent microscopy, and Fourier transform infrared spectroscopy. Efficiency of the RML-CS/CNWs was compared to the free RML to synthesize eugenyl benzoate for parameters: reaction temperature, stirring rate, reusability, and thermal stability. Under optimal experimental conditions (50°C, 250 rpm, catalyst loading 3 mg/mL), a twofold increase in yield of eugenyl benzoate was observed for RML-CS/CNWs as compared to free RML, with the former achieving maximum yield of the ester at 62.1% after 5 hr. Results demonstrated that the strategy adopted to prepare RML-CS/CNWs was useful, producing an improved and prospectively greener biocatalyst that supported a sustainable process to prepare eugenyl benzoate. Moreover, RML-CS/CNWs are biodegradable and perform esterification reactions under ambient conditions as compared to the less eco-friendly conventional acid catalyst. This research provides a facile and promising approach for improving activity of RML in which the resultant RML-CS/CNWs demonstrated good operational stability for up to eight successive esterification cycles to synthesize eugenyl benzoate.
以壳聚糖-甲壳素纳米晶须（CS/CNWs）制备了一种替代性的环境友好型载体，用于共价固定米黑根毛霉脂肪酶（RML），以提高RML在合成苯甲酸丁香酯中的操作稳定性和可回收性。使用X射线衍射、荧光显微镜和傅里叶变换红外光谱对CS/CNWs载体和RML-CS/CNWs进行了表征。将RML-CS/CNWs的效率与游离RML进行比较，以合成苯甲酸丁香酯，考察参数包括：反应温度、搅拌速率、可重复使用性和热稳定性。在最佳实验条件（50°C，250 rpm，催化剂负载量3 mg/mL）下，与游离RML相比，RML-CS/CNWs合成的苯甲酸丁香酯产率提高了两倍，前者在5小时后酯的最大产率达到62.1%。结果表明，制备RML-CS/CNWs所采用的策略是有效的，产生了一种改进的、前景更绿色的生物催化剂，支持了一种可持续的苯甲酸丁香酯制备工艺。此外，与不太环保的传统酸催化剂相比，RML-CS/CNWs可生物降解，并在环境条件下进行酯化反应。本研究为提高RML的活性提供了一种简便且有前景的方法，其中所得的RML-CS/CNWs在连续八次酯化循环中表现出良好的操作稳定性，用于合成苯甲酸丁香酯。
