Department of Chemistry, Institute of Chemical Technology, Mumbai, 400019, India.
Department of Chemistry, Pratap College of Arts, Science and Commerce, Amalner, 425401, India.
Bioprocess Biosyst Eng. 2017 Oct;40(10):1463-1478. doi: 10.1007/s00449-017-1804-0. Epub 2017 Jul 4.
The present work deals with the designing of biocompatible hybrid blend of cellulosic copolymers made of hydroxypropyl methylcellulose (HMC) and chitosan (CHI) for immobilization of Rhizomucor miehei lipase (RML), in order to construct the robust biocatalytic system to synthesize industrially important dodecanoate compounds (fatty acid esters). The present biocatalyst HMC:CHI:RML was characterized in detail by various physical and biochemical methods and subsequently applied for the synthesis of fatty acid esters. The protocol was optimized in detail with kinetic parameters which provides excellent % conversion, and further we have synthesized fifteen industrially important compounds which have wide potential for commercial applications. The immobilized lipase HMC:CHI:RML offered four- to eightfold higher conversion and biocatalytic activity as compared to crude lipase. Besides this, recyclability study was also performed to assess economic and industrial viability.
本工作涉及设计羟丙基甲基纤维素（HMC）和壳聚糖（CHI）组成的纤维素共聚物的生物相容性杂化共混物，用于固定里氏木霉脂肪酶（RML），以构建用于合成工业上重要的月桂酸酯化合物（脂肪酸酯）的稳健的生物催化体系。详细的各种物理和生化方法对 present 生物催化剂 HMC:CHI:RML 进行了表征，随后将其用于合成脂肪酸酯。详细优化了该方案，提供了出色的转化率参数，进一步合成了十五种具有广泛商业应用潜力的工业重要化合物。与粗脂肪酶相比，固定化脂肪酶 HMC:CHI:RML 提供了四到八倍的更高转化率和生物催化活性。除此之外，还进行了可回收性研究，以评估经济和工业可行性。
