Lypge在斑马鱼眼睛和松果体形成中的作用。

Involvement of Lypge in the formation of eye and pineal gland in zebrafish.

作者信息

Ji Dongrui, Wang Su, Li Mingyue, Zhang Shicui, Li Hongyan

机构信息

Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Evolution & Development, Department of Marine Biology, Ocean University of China, Qingdao 266003, China.

出版信息

Gene. 2018 Feb 5;642:491-497. doi: 10.1016/j.gene.2017.11.062. Epub 2017 Nov 28.

DOI:10.1016/j.gene.2017.11.062

PMID:29196253

Abstract

The proteins of Ly-6 (lymphocyte antigen-6) family are involved in the regulation of immunoreaction, cell migration and adhesion, and neuronal excitability. However, little is known about the function of Ly-6 proteins in embryogenesis. Herein, we identified a GPI anchored Ly-6 member named ly6 expressed in pineal gland and eye (lypge). Dynamic expression pattern of lypge was revealed by whole mount in situ hybridization. It was strikingly expressed in the pineal gland and cone photoreceptor, and its expression was regulated by orthodenticle homolog 5 (otx5) which has been shown to control the expression of many pineal genes. In addition, we demonstrated that lypge was rhythmically expressed in larvae from 4dpf on. Moreover, knockdown of lypge resulted in small head and small eye formed in zebrafish embryos. These suggest that Lypge is involved in the formation of the eye and pineal gland in early development of zebrafish.

摘要

Ly-6（淋巴细胞抗原-6）家族蛋白参与免疫反应、细胞迁移与黏附以及神经元兴奋性的调节。然而，关于Ly-6蛋白在胚胎发育中的功能却知之甚少。在此，我们鉴定出一种在松果体和眼睛中表达的糖基磷脂酰肌醇（GPI）锚定的Ly-6成员，命名为ly6（lypge）。通过整体原位杂交揭示了lypge的动态表达模式。它在松果体和视锥光感受器中显著表达，并且其表达受正齿同源物5（otx5）调控，otx5已被证明可控制许多松果体基因的表达。此外，我们证明lypge在4天胚胎期（dpf）后的幼虫中呈节律性表达。而且，敲低lypge会导致斑马鱼胚胎出现小头和小眼睛。这些表明Lypge参与斑马鱼早期发育中眼睛和松果体的形成。