Xu Dan-Dan, Li Xiao-Feng, Li Yu-Huan, Liu Yan-Hui, Huang Cheng, Meng Xiao-Ming, Li Jun
Anhui Province Key Laboratory of Major Autoimmune Diseases, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei 230032, China; Pharmacy Department, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China; The Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, Hefei 230032, China; Institute for Liver Diseases of Anhui Medical University, Hefei 230032, China.
Anhui Province Key Laboratory of Major Autoimmune Diseases, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei 230032, China; The Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, Hefei 230032, China; Institute for Liver Diseases of Anhui Medical University, Hefei 230032, China.
Biochem Biophys Res Commun. 2018 Mar 25;498(1):199-206. doi: 10.1016/j.bbrc.2017.11.178. Epub 2017 Dec 2.
TIPE2, the tumor necrosis factor (TNF)-α-induced protein 8-like 2 (TNFAIP8L2), plays an important role in regulating inflammation and immune homeostasis. Recent studies discovered that TIPE-2 involved in the development of several tumors and other proliferative diseases. The purpose of this study was to explore the function of TIPE-2 in the activation and proliferation in HSC-T6 cells. Our study showed low expression of TIPE-2 in primary HSCs from CCl4-treated mice and activated HSC-T6 cells. Functionally, over-expression of TIPE-2 by GV141-TIPE-2 hindered the HSC-T6 cells activation and proliferation and expressions of β-Catenin, Cmyc, Cyclin D1. However, inhibition TIPE-2 expression by TIPE-2 siRNA showed the opposite effect. These observations revealed that TIPE-2 held a protective effect on liver fibrosis and could be a potential therapeutic target.
TIPE2，即肿瘤坏死因子（TNF）-α诱导蛋白8样2（TNFAIP8L2），在调节炎症和免疫稳态中发挥重要作用。最近的研究发现，TIPE-2参与了多种肿瘤及其他增殖性疾病的发生发展。本研究旨在探讨TIPE-2在肝星状细胞（HSC）-T6细胞激活和增殖中的作用。我们的研究表明，在四氯化碳处理小鼠的原代肝星状细胞及激活的HSC-T6细胞中，TIPE-2表达较低。在功能上，通过GV141-TIPE-2过表达TIPE-2可抑制HSC-T6细胞的激活、增殖以及β-连环蛋白、Cmyc、细胞周期蛋白D1的表达。然而，用TIPE-2小干扰RNA抑制TIPE-2表达则产生相反的效果。这些观察结果表明，TIPE-2对肝纤维化具有保护作用，可能是一个潜在的治疗靶点。
