Shin Joong Ho, Hong Jisoo, Go Hyeyun, Park Juhwan, Kong Minsuk, Ryu Sangryeol, Kim Kwang-Pyo, Roh Eunjung, Park Je-Kyun
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration , Wanju-gun 55365, Republic of Korea.
J Agric Food Chem. 2018 Jan 10;66(1):290-297. doi: 10.1021/acs.jafc.7b03582. Epub 2017 Dec 19.
This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 × 10 CFU of E. coli O157:H7 and 1.47 × 10 CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.
本文介绍了一种手持设备，该设备能够简化多步骤检测，以实现对食源性病原体的灵敏检测。该设备能够对大肠杆菌O157:H7、鼠伤寒沙门氏菌、金黄色葡萄球菌和蜡样芽孢杆菌进行多重检测。对每种细菌的检测限进行了表征，然后针对两种具有代表性的食源性病原体展示了从受污染的新鲜生菜中检测细菌的情况。我们采用了一种样品预处理方案，从受污染的生菜中回收并浓缩目标细菌，无需富集过程即可检测到每克生菜中1.87×10 CFU的大肠杆菌O157:H7和1.47×10 CFU的鼠伤寒沙门氏菌。最后，我们证明，通过在预处理前将受污染的生菜在生长培养基中富集6小时，大肠杆菌O157:H7和鼠伤寒沙门氏菌的检测限可降低至每克生菜1 CFU。
