Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Chandigarh 160036, India.
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun 248002, India.
Biosensors (Basel). 2023 Feb 9;13(2):249. doi: 10.3390/bios13020249.
The detection of pathogens in food substances is of crucial concern for public health and for the safety of the natural environment. Nanomaterials, with their high sensitivity and selectivity have an edge over conventional organic dyes in fluorescent-based detection methods. Advances in microfluidic technology in biosensors have taken place to meet the user criteria of sensitive, inexpensive, user-friendly, and quick detection. In this review, we have summarized the use of fluorescence-based nanomaterials and the latest research approaches towards integrated biosensors, including microsystems containing fluorescence-based detection, various model systems with nano materials, DNA probes, and antibodies. Paper-based lateral-flow test strips and microchips as well as the most-used trapping components are also reviewed, and the possibility of their performance in portable devices evaluated. We also present a current market-available portable system which was developed for food screening and highlight the future direction for the development of fluorescence-based systems for on-site detection and stratification of common foodborne pathogens.
食品中病原体的检测对公众健康和自然环境安全至关重要。与传统的有机染料相比，纳米材料在基于荧光的检测方法中具有更高的灵敏度和选择性。生物传感器中微流控技术的进步满足了用户对灵敏、廉价、易用和快速检测的要求。在这篇综述中，我们总结了基于荧光的纳米材料的应用以及集成生物传感器的最新研究方法，包括含有荧光检测的微系统、各种纳米材料模型系统、DNA 探针和抗体。还回顾了纸基横向流动测试条和微芯片以及最常用的捕获组件，并评估了它们在便携式设备中的性能的可能性。我们还展示了一种目前市场上可用于食品筛选的便携式系统，并强调了开发基于荧光的现场检测和常见食源性病原体分层系统的未来方向。
