通过基于斑马鱼（Danio rerio）胚胎的卵黄蛋白原1（vtg1）mRNA表达评估内分泌干扰化学物质（EDCs）的雌激素和抗雌激素作用。
Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtg1) mRNA expression.
作者信息
Chen Minjie, Zhang Jie, Pang Shaochen, Wang Chang, Wang Ling, Sun Yonghua, Song Maoyong, Liang Yong
机构信息
School of Medicine, Jianghan University, Wuhan 430056, PR China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, PR China.
College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, PR China.
出版信息
Comp Biochem Physiol C Toxicol Pharmacol. 2018 Jan;204:45-50. doi: 10.1016/j.cbpc.2017.11.010. Epub 2017 Dec 1.
By measuring the vitellogenin 1 (vtg1) expression through quantitative PCR and in situ hybridization, we used the zebrafish embryo as an in vivo model to access the estrogenic or anti-estrogenic effects of several endocrine disrupting chemicals (EDCs), such as natural estrogen 17β-estradiol (E2), estriol (E3), synthetic hormones including diethylstilbestrol (DES), 4-octyl phenol (OP), bisphenol A (BPA), tamoxifen (TMX) and 3-(2,3-dibromopropyl) isocyanurate (TBC). According to our data, the estrogenic effect of the tested chemicals was ranked as: DES>E>E>OP>BPA, which is consistent with various in vivo and in vitro models. Therefore, the measurement of vtg1 gene expression in zebrafish embryos would be a valuable method for screening EDCs including both environmental estrogens and anti-estrogens.
通过定量PCR和原位杂交测量卵黄蛋白原1（vtg1）的表达，我们将斑马鱼胚胎用作体内模型，以评估几种内分泌干扰化学物质（EDC）的雌激素或抗雌激素作用，这些物质包括天然雌激素17β-雌二醇（E2）、雌三醇（E3）、合成激素如己烯雌酚（DES）、4-辛基苯酚（OP）、双酚A（BPA）、他莫昔芬（TMX）和3-（2,3-二溴丙基）异氰脲酸酯（TBC）。根据我们的数据，所测试化学物质的雌激素作用排序为：DES>E>E>OP>BPA，这与各种体内和体外模型一致。因此，测量斑马鱼胚胎中vtg1基因的表达将是一种筛选包括环境雌激素和抗雌激素在内的EDC的有价值方法。
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