Ando Jin, Saito Motonobu, Imai Jun-Ichi, Ito Emi, Yanagisawa Yuka, Honma Reiko, Saito Katsuharu, Tachibana Kazunoshin, Momma Tomoyuki, Ohki Shinji, Ohtake Tohru, Watanabe Shinya, Waguri Satoshi, Kono Koji, Takenoshita Seiichi
Department of Gastrointestinal Tract Surgery, Fukushima Medical University School of Medicine.
Medical-Industrial Translational Research Center, Fukushima Medical University.
Fukushima J Med Sci. 2017 Dec 19;63(3):141-151. doi: 10.5387/fms.2017-08. Epub 2017 Dec 1.
The T-box 19 (TBX19) gene encodes a transcription factor characterized by a highly conserved DNA-binding motif (T-box). Recent studies have revealed that TBX19 has been identified as one of the genes activated by KRAS mutations, and is upregulated in colon adenoma. These results indicate that TBX19 may work as an oncogene in colorectal cancer (CRC). However, the expression and role of TBX19 have yet to be investigated. Here, we investigated TBX19 mRNA and protein expressions in colon cancer cells or surgically resected CRC. We found that TBX19 mRNA expression was significantly increased in tumorous tissues compared to that in non-tumorous tissues, and increased TBX19 mRNA expression was associated with positive lymph node metastasis in our cohort. The expression of TBX19 mRNA was not correlated with that of TBX19 protein in tissue sample taken from the CRC patients. Moreover, TBX19 showed positive staining even in the normal colonic tissues and the adjacent non-tumorous tissues. These results suggest that the expression of TBX19 protein is not correlated with the expression of TBX19 mRNA. In addition, our results promote further investigations into the impact of TBX19 upregulation on colorectal carcinogenesis, as well as the underlying mechanisms.
T 盒 19（TBX19）基因编码一种转录因子，其特征在于具有高度保守的 DNA 结合基序（T 盒）。最近的研究表明，TBX19 已被确定为受 KRAS 突变激活的基因之一，且在结肠腺瘤中上调。这些结果表明，TBX19 在结直肠癌（CRC）中可能作为一种癌基因发挥作用。然而，TBX19 的表达及作用尚未得到研究。在此，我们研究了结肠癌细胞或手术切除的 CRC 中 TBX19 的 mRNA 和蛋白表达。我们发现，与非肿瘤组织相比，肿瘤组织中 TBX19 的 mRNA 表达显著增加，并且在我们的队列中，TBX19 mRNA 表达增加与阳性淋巴结转移相关。在 CRC 患者的组织样本中，TBX19 mRNA 的表达与 TBX19 蛋白的表达不相关。此外，即使在正常结肠组织和相邻的非肿瘤组织中，TBX19 也呈阳性染色。这些结果表明，TBX19 蛋白的表达与 TBX19 mRNA 的表达不相关。此外，我们的结果促进了对 TBX19 上调对结直肠癌发生的影响及其潜在机制的进一步研究。
