Van Wychen S, Laurens L M L
National Renewable Energy Laboratory, National Bioenergy Center, Golden, CO, USA.
Methods Mol Biol. 2020;1980:203-214. doi: 10.1007/7651_2017_107.
The aliphatic chains of fatty acids are the most prominent and potentially the highest value precursor constituents of algal biomass, and thus accurately quantifying the algal biomass total fatty acid content is a prerequisite for comparing algal strains, growth conditions, and processes. Direct, acid-catalyzed transesterification of whole microalgal biomass is a simple, effective, and widely used method to determine the fatty acid content in whole algal biomass. Such a direct transesterification procedure typically covers the following steps: first, solubilizing the lipids in the biomass matrix and then liberating the fatty acids to make these available for catalytic upgrading to fatty acid methyl esters (FAMEs), subsequent extraction into hexane, and then quantification by gas chromatography. The method we describe here requires less than 10 mg of biomass per sample and is considered high-throughput and highly accurate.
脂肪酸的脂肪链是藻类生物质中最突出且可能具有最高价值的前体成分，因此准确量化藻类生物质中的总脂肪酸含量是比较藻类菌株、生长条件和工艺的前提。对整个微藻生物质进行直接的酸催化酯交换反应是一种简单、有效且广泛使用的方法，用于测定整个藻类生物质中的脂肪酸含量。这种直接酯交换程序通常包括以下步骤：首先，将生物质基质中的脂质溶解，然后释放脂肪酸，使其可用于催化升级为脂肪酸甲酯（FAMEs），随后萃取到己烷中，然后通过气相色谱法定量。我们在此描述的方法每个样品所需的生物质少于10毫克，被认为是高通量且高度准确的。
