通过体内给予麻醉剂和惊厥剂对小鼠大脑中特定神经元蛋白磷酸化状态的调节。
Regulation of the state of phosphorylation of specific neuronal proteins in mouse brain by in vivo administration of anesthetic and convulsant agents.
作者信息
Strömbom U, Forn J, Dolphin A C, Greengard P
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4687-90. doi: 10.1073/pnas.76.9.4687.
Abstract
The effect of drug treatment in vivo on the state of phosphorylation of two specific neuronal proteins, proteins Ia and Ib, has been studied in mouse brain. For this purpose, animals were killed by immersion into liquid nitrogen, and proteins Ia and Ib were extracted by a procedure designed to prevent alterations in their state of phosphorylation. Several anesthetic agents (pentobarbital, chloral hydrate, and urethane) each caused a decrease in the state of phosphorylation of these proteins. Conversely, the convulsant agents pentylenetetrazol and picrotoxin each caused an increase in the state of phosphorylation of these proteins. Neither the anesthetic nor the convulsant agents affected the total amount of these proteins. The results are compatible with a role for proteins Ia and Ib in neuronal function.
摘要
已在小鼠大脑中研究了体内药物治疗对两种特定神经元蛋白（蛋白Ia和蛋白Ib）磷酸化状态的影响。为此，将动物浸入液氮中处死，并用一种旨在防止其磷酸化状态改变的方法提取蛋白Ia和蛋白Ib。几种麻醉剂（戊巴比妥、水合氯醛和乌拉坦）均导致这些蛋白的磷酸化状态降低。相反，惊厥剂戊四氮和印防己毒素均导致这些蛋白的磷酸化状态增加。麻醉剂和惊厥剂均未影响这些蛋白的总量。这些结果与蛋白Ia和蛋白Ib在神经元功能中的作用相符。
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