• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种VI型胶原相关糖多肽是猪皮肤中主要的伴刀豆球蛋白A结合成分。

A type VI collagen-related glycopolypeptide is the major concanavalin A-binding component in pig skin.

作者信息

King I A, Tabiowo A, Fryer P R, Pope F M

机构信息

Dermatology Research Group, M.R.C. Clinical Research Centre, Harrow, Middx., U.K.

出版信息

Biochem J. 1989 Jan 1;257(1):79-86. doi: 10.1042/bj2570079.

DOI:10.1042/bj2570079
PMID:2920030
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1135540/
Abstract

The major concanavalin A-binding component in urea/deoxycholate/mercaptoethanol extracts of pig skin was a collagenous disulphide-cross-linked glycopolypeptide with an apparent molecular mass of 150 kDa and a pI of 5.5. Antiserum against the electrophoretically purified glycopolypeptide gave strong dermal staining similar to that seen with fluorescent concanavalin A. Immunocytochemical labelling showed prominent labelling of 3-4 nm dermal microfilaments, particularly those associated with dermal blood vessels and mast cells. Immunoblotting with authentic antiserum indicated that the major skin glycopolypeptide was probably identical with collagen-like glycoprotein, the tissue form of the alpha 1/alpha 2 subunits of type VI collagen. This was confirmed by immunoblotting of authentic type VI collagen from pepsin-treated pig skin. Immunoblotting, metabolic labelling with [3H]glucosamine and immune precipitation showed that an immunoreactive collagenous glycopolypeptide was synthesized and secreted by cultured pig skin fibroblasts. The results suggest that type VI collagen is the major concanavalin A-binding component in pig skin.

摘要

猪皮尿素/脱氧胆酸盐/巯基乙醇提取物中主要的伴刀豆球蛋白A结合成分是一种胶原性二硫键交联糖多肽，其表观分子量为150 kDa，pI为5.5。针对经电泳纯化的糖多肽的抗血清产生了强烈的真皮染色，类似于用荧光伴刀豆球蛋白A观察到的染色。免疫细胞化学标记显示3 - 4 nm真皮微丝有明显标记，特别是那些与真皮血管和肥大细胞相关的微丝。用真实抗血清进行免疫印迹表明，主要的皮肤糖多肽可能与VI型胶原α1/α2亚基的组织形式——类胶原糖蛋白相同。用胃蛋白酶处理的猪皮中的真实VI型胶原进行免疫印迹证实了这一点。免疫印迹、用[3H]葡糖胺进行代谢标记和免疫沉淀表明，培养的猪皮肤成纤维细胞合成并分泌了一种免疫反应性胶原性糖多肽。结果表明，VI型胶原是猪皮中主要的伴刀豆球蛋白A结合成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/4d19a508d539/biochemj00216-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/6ff2926f940f/biochemj00216-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/59cde80aae9a/biochemj00216-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/1e722fb95d64/biochemj00216-0087-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/528466569e66/biochemj00216-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/ab639858cb0d/biochemj00216-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/ce943c71e4e7/biochemj00216-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/20da7d92e777/biochemj00216-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/4d19a508d539/biochemj00216-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/6ff2926f940f/biochemj00216-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/59cde80aae9a/biochemj00216-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/1e722fb95d64/biochemj00216-0087-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/528466569e66/biochemj00216-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/ab639858cb0d/biochemj00216-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/ce943c71e4e7/biochemj00216-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/20da7d92e777/biochemj00216-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/471b/1135540/4d19a508d539/biochemj00216-0091-a.jpg

相似文献

1
A type VI collagen-related glycopolypeptide is the major concanavalin A-binding component in pig skin.一种VI型胶原相关糖多肽是猪皮肤中主要的伴刀豆球蛋白A结合成分。
Biochem J. 1989 Jan 1;257(1):79-86. doi: 10.1042/bj2570079.
2
Evidence that major 78-44-kD concanavalin A-binding glycopolypeptides in pig epidermis arise from the degradation of desmosomal glycoproteins during terminal differentiation.有证据表明，猪表皮中主要的78 - 44kD伴刀豆球蛋白A结合糖多肽是在终末分化过程中桥粒糖蛋白降解产生的。
J Cell Biol. 1987 Dec;105(6 Pt 2):3053-63. doi: 10.1083/jcb.105.6.3053.
3
A lectin-binding glycoprotein of Mr 135,000 associated with basal keratinocytes in pig epidermis.一种分子量为135,000的凝集素结合糖蛋白，与猪表皮的基底角质形成细胞相关。
Biochem J. 1986 Jul 15;237(2):405-14. doi: 10.1042/bj2370405.
4
Isolation from bovine elastic tissues of collagen type VI and characterization of its form in vivo.从牛弹性组织中分离出VI型胶原蛋白并对其体内形态进行表征。
Biochem J. 1985 Sep 1;230(2):465-74. doi: 10.1042/bj2300465.
5
Collagen gene expression by cultured human skin fibroblasts. Abundant steady-state levels of type VI procollagen messenger RNAs.培养的人皮肤成纤维细胞的胶原蛋白基因表达。VI型前胶原信使核糖核酸的丰富稳态水平。
J Clin Invest. 1989 Mar;83(3):791-5. doi: 10.1172/JCI113959.
6
Molecular composition of type VI collagen. Evidence for chain heterogeneity in mammalian tissues and cultured cells.VI型胶原蛋白的分子组成。哺乳动物组织和培养细胞中链异质性的证据。
Biochem J. 1990 Dec 15;272(3):787-95. doi: 10.1042/bj2720787.
7
Characterization of a type VI collagen-related Mr-140 000 protein from cutis-laxa fibroblasts in culture.培养的皮肤松弛症成纤维细胞中一种与VI型胶原相关的140000 Mr蛋白质的特性分析。
Biochem J. 1985 Apr 15;227(2):491-502. doi: 10.1042/bj2270491.
8
Abnormal formation of collagen cross-links in skin fibroblasts cultured from patients with Ehlers-Danlos syndrome type VI.从患有Ⅵ型埃勒斯-当洛综合征患者培养的皮肤成纤维细胞中胶原蛋白交联的异常形成。
Proc Assoc Am Physicians. 1997 Jan;109(1):33-41.
9
Type VI collagen and glycoprotein MFPI are distinct components of the extracellular matrix.VI型胶原蛋白和糖蛋白MFPI是细胞外基质的不同组成成分。
Biochem J. 1986 May 15;236(1):299-302. doi: 10.1042/bj2360299.
10
Partial characterization of an unusual 185 kDa protein synthesized by dermal fibroblasts from patients with Marfan syndrome: identification of the protein as type IV collagen.对马凡氏综合征患者皮肤成纤维细胞合成的一种异常185 kDa蛋白质的部分特性分析：鉴定该蛋白质为IV型胶原。
J Biochem. 1989 Sep;106(3):490-4. doi: 10.1093/oxfordjournals.jbchem.a122879.

引用本文的文献

1
150-kD von Willebrand factor binding protein extracted from human vascular subendothelium is type VI collagen.从人血管内皮下层提取的150-kD血管性血友病因子结合蛋白是VI型胶原。
J Clin Invest. 1991 Jul;88(1):253-9. doi: 10.1172/JCI115285.
2
Molecular composition of type VI collagen. Evidence for chain heterogeneity in mammalian tissues and cultured cells.VI型胶原蛋白的分子组成。哺乳动物组织和培养细胞中链异质性的证据。
Biochem J. 1990 Dec 15;272(3):787-95. doi: 10.1042/bj2720787.

本文引用的文献

1
Isolation of a unique collagenous fraction from limited pepsin digests of human placental tissue. Characterization of one of the constituent polypeptide chains.从人胎盘组织的有限胃蛋白酶消化物中分离出一种独特的胶原成分。其中一种组成多肽链的特性分析。
J Biol Chem. 1980 Jan 10;255(1):290-5.
2
Differential lectin binding to cellular membranes in the epidermis of the newborn rat.新生大鼠表皮中不同凝集素与细胞膜的结合
Proc Natl Acad Sci U S A. 1980 Jan;77(1):477-9. doi: 10.1073/pnas.77.1.477.
3
Structural diversity and domain composition of a unique collagenous fragment (intima collagen) obtained from human placenta.
从人胎盘中获得的一种独特胶原片段（内膜胶原）的结构多样性和结构域组成
Biochem J. 1983 May 1;211(2):295-302. doi: 10.1042/bj2110295.
4
Alterations in membrane sugars during epidermal differentiation: visualization with lectins and role of glycosidases.表皮分化过程中膜糖的变化：用凝集素进行可视化及糖苷酶的作用
J Histochem Cytochem. 1983 Jul;31(7):887-97. doi: 10.1177/31.7.6854004.
5
Use of lectins for the study of human epidermal differentiation.凝集素在人类表皮分化研究中的应用。
Br J Dermatol. 1982 Nov;107 Suppl 23:143-6. doi: 10.1111/j.1365-2133.1982.tb01047.x.
6
A cysteine-rich collagenous protein from bovine placenta. Isolation of its constituent polypeptide chains and some properties of the non-denatured protein.一种来自牛胎盘的富含半胱氨酸的胶原蛋白。其组成多肽链的分离及非变性蛋白的一些特性。
Eur J Biochem. 1981;114(1):17-25.
7
The role of intermolecular disulfide bonding in deposition of GP140 in the extracellular matrix.分子间二硫键在GP140于细胞外基质中沉积的作用。
J Cell Biol. 1984 Jul;99(1 Pt 1):105-14. doi: 10.1083/jcb.99.1.105.
8
Distribution of CL glycoprotein in tissues: an immunohistochemical study.CL糖蛋白在组织中的分布：一项免疫组织化学研究。
Coll Relat Res. 1983 Nov;3(6):469-88. doi: 10.1016/s0174-173x(83)80027-2.
9
A collagen-like glycoprotein of the extracellular matrix is the undegraded form of type VI collagen.细胞外基质中的一种类胶原蛋白是VI型胶原蛋白的未降解形式。
Biochemistry. 1984 Jul 31;23(16):3675-81. doi: 10.1021/bi00311a016.
10
Immunochemistry, genuine size and tissue localization of collagen VI.胶原蛋白VI的免疫化学、真实大小及组织定位
Eur J Biochem. 1984 Aug 1;142(3):493-502. doi: 10.1111/j.1432-1033.1984.tb08313.x.