Liberatore F A, Comeau R D, Lawton R G
Medical Products Department, E.I. du Pont de Nemours & Company, Billerica, MA 01862.
Biochem Biophys Res Commun. 1989 Feb 15;158(3):640-5. doi: 10.1016/0006-291x(89)92769-1.
The Cyssor reagent, 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide, which will cleave a protein chain at Cys under acidic conditions, cross-linked unreduced and partially reduced antibody at pH 8.0. No cleavage of the antibody occurred suggesting that the Cyssor reagent may be useful with certain proteins as a heterobifunctional cross-linker.
西索尔试剂，即2-甲基-N1-苯磺酰基-N4-溴乙酰基醌二亚胺，在酸性条件下可在半胱氨酸处切割蛋白质链，在pH 8.0时可使未还原和部分还原的抗体发生交联。抗体未发生切割，这表明西索尔试剂作为一种异双功能交联剂可能对某些蛋白质有用。
