Krajci P, Solberg R, Sandberg M, Oyen O, Jahnsen T, Brandtzaeg P
Laboratory for Immunohistochemistry and Immunopathology, and University of Oslo, Rikshospitalet, Norway.
Biochem Biophys Res Commun. 1989 Feb 15;158(3):783-9. doi: 10.1016/0006-291x(89)92790-3.
A 2.5 kilobase (kb) cDNA clone containing 92% of the coding region for human transmembrane secretory component (SC) or poly-Ig receptor, was isolated from a mammary gland cDNA library. The cDNA clone encoded a protein of 693 amino acids which showed 99% homology with the primary amino acid sequence of human free SC as reported by Eiffert et al. (1), and 54% homology with the deduced amino acid sequence of rabbit transmembrane SC for which cDNA was cloned by Mostov et al. (2). Northern blot analysis showed mRNA expression in various human exocrine tissues in good agreement with our previous immunohistochemical studies of SC.
从乳腺cDNA文库中分离出一个2.5千碱基（kb）的cDNA克隆，它包含人跨膜分泌成分（SC）或多聚免疫球蛋白受体编码区的92%。该cDNA克隆编码一个693个氨基酸的蛋白质，与Eiffert等人（1）报道的人游离SC的一级氨基酸序列有99%的同源性，与Mostov等人（2）克隆出cDNA的兔跨膜SC的推导氨基酸序列有54%的同源性。Northern印迹分析显示，mRNA在各种人类外分泌组织中的表达与我们之前对SC的免疫组织化学研究结果高度一致。
