编码大鼠肝脏尿酸氧化酶的全长cDNA克隆的核苷酸序列。

The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase.

作者信息

Alvares K, Nemali M R, Reddy P G, Wang X D, Rao M S, Reddy J K

机构信息

Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611.

出版信息

Biochem Biophys Res Commun. 1989 Feb 15;158(3):991-5. doi: 10.1016/0006-291x(89)92820-9.

DOI:10.1016/0006-291x(89)92820-9

PMID:2920046

Abstract

Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a lambda gt11 cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a lambda gt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3'-untranslated and 41 bp of 5'-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3' end.

摘要

最近我们报道了从λgt11 cDNA文库中分离出的一个cDNA克隆（pUOX - 1）的序列，它编码大鼠肝脏尿酸氧化酶（EC 1.7.3.3），但这个克隆缺少该酶N端区域的编码核苷酸序列。利用pUOX - 1克隆的cDNA插入片段作为探针，我们现在通过噬菌斑杂交从λgt10文库中分离出了一个全长cDNA克隆pUOX - 2。对pUOX - 2克隆的核苷酸序列分析表明，它有1379个碱基对，一个开放阅读框编码303个氨基酸残基，对应分子量为34931道尔顿。除了开放阅读框外，pUOX - 2还包含439 bp的3'非翻译序列和41 bp的5'非翻译序列。在3'端，共有多聚腺苷酸化信号AATAAA位于一段多聚（A）+残基之前。