Li Ting Yu, Huo Xiao Kui, Zheng Lu, Wang Chao, Cong Hai Jian, Xiang Ting, Zhang Lin, Zhang Bao Jing, Huang Shan Shan, Wu Bin, Li Xin Yu
Department of Pharmacy, Dalian Children's Hospital, Dalian, China.
Academy of Integrative Medicine, College of Pharmacy, Dalian Medical University, Dalian, China.
Pharmacogn Mag. 2017 Oct-Dec;13(52):566-570. doi: 10.4103/pm.pm_81_17. Epub 2017 Nov 13.
Chaiqin Qingning Capsule (CQQNC) was a prescription of Traditional Chinese Medicine with the effects of clearing away heat and removing toxin, harmonizing the exterior and interior, it was widely used in Asian, for example, China and Japan, different batches of the raws materials and different processing time may be the vital factor which raised a challenge to control the quality of the CQQNC.
In this experiment, a high-performance liquid chromatography-mass spectrometry/MS (HPLC-MS/MS) method was developed to simultaneously determine ten bioactive components for the quality control of CQQNC. Chromatographic separation was achieved using an XBridge BEH C18 column (150 mm × 4.6 mm, 2.5 μm) with a mobile phase composed of 10 mm aqueous ammonium acetate and acetonitrile using a gradient elution in 20 min. This study was conducted by multiple reaction monitoring mode through electrospray ionization resource with a negative ionization mode.
The established method was validated with good performance of precision, accuracy, stability, and reproducibility and was utilized to simultaneously quantify ten constituents of CQQNC obtained from seven different batches.
It is the first time to report the rapid and simultaneous analysis of the ten compounds in CQQNC by HPLC-MS/MS and apply to determine 10 constituents in 7 batches of CQQNC bought from drug store in china. This method could be considered as good quality criteria to control the quality of CQQNC.
In this paper, a simple, specific, and rapid high-performance liquid chromatogram coupled with triple-quadrupole mass spectrometry method for simultaneous quantification of ten constituents in Chaiqin Qingning Capsule has been developed for the first time. This method could be considered as good quality criteria to control the quality of CQQNC. CHM: Chinese herbal medicine; TCM: Traditional Chinese Medicine; CQQNC: Triple-quadrupole mass spectrometry Chaiqin Qingning Capsules; HPLC-MS/MS: High liquid chromatography equipped with tandem mass spectrometry; ESI: Electrospray ionization; DP: Declustering potential; CE: Collision energy; RSD: Relative standard deviation; LOD: Limit of detection; LOQ: Limit of quantity.
柴芩清宁胶囊（CQQNC）是一种具有清热解毒、表里双解功效的中药方剂，在亚洲如中国和日本广泛使用，不同批次的原材料和不同的加工时间可能是控制柴芩清宁胶囊质量的关键因素，这对其质量控制提出了挑战。
本实验建立了一种高效液相色谱 - 质谱联用/质谱（HPLC - MS/MS）方法，用于同时测定柴芩清宁胶囊质量控制中的十种生物活性成分。采用XBridge BEH C18柱（150 mm×4.6 mm，2.5μm）进行色谱分离，流动相由10 mM乙酸铵水溶液和乙腈组成，在20分钟内进行梯度洗脱。本研究通过电喷雾电离源在负离子模式下的多反应监测模式进行。
所建立的方法经精密度、准确度、稳定性和重现性验证，性能良好，并用于同时定量分析来自七个不同批次的柴芩清宁胶囊的十种成分。
首次报道了采用HPLC - MS/MS快速同时分析柴芩清宁胶囊中的十种化合物，并应用于测定从中国药店购买的7批次柴芩清宁胶囊中的10种成分。该方法可作为控制柴芩清宁胶囊质量的良好质量标准。
本文首次建立了一种简单、特异、快速的高效液相色谱 - 三重四极杆质谱联用方法，用于同时定量分析柴芩清宁胶囊中的十种成分。该方法可作为控制柴芩清宁胶囊质量的良好质量标准。CHM：中草药；TCM：传统中药；CQQNC：柴芩清宁胶囊；HPLC - MS/MS：配备串联质谱的高效液相色谱；ESI：电喷雾电离；DP：去簇电位；CE：碰撞能量；RSD：相对标准偏差；LOD：检测限；LOQ：定量限。
