Evaluation of a Tc-tricine Vascular Disrupting Agent as an Imaging in 4T1 Mouse Breast Tumor Model.

Colchicine as a vascular disrupting agent creates microtubule destabilization which induces vessel blockage and consequently cell death. Accordingly, colchicines and its analogues radiolabeled with Tc may have potential for visualization of tumor. In this work, deacetylcolchicine a colchicine analogue was labeled with Tc via tricine as a coligand and characterized for its tumor targeting properties. The radiochemical stability and the biodistribution were studied in 4T1 breast tumor model bearing mice. Labeling yield of more than 90% was obtained corresponding to a specific activity of 46 MBq/µmol. biodistribution studies demonstrated that radiocomplex had high tumor to muscle and tumor to blood ratios at early time points. Planer gamma imaging of tumor bearing mice showed that this radioconjugate was able to clearly visualize tumors. According to high tumor uptake, presented radiocomplex may have a potential for targeted imaging studies.