Pourahmad Jaktaji Razieh, Zargampoor Farzaneh
Faculty of Science and Biotechnology Center, University of Shahrekord, Iran.
Faculty of Science, Shaheed Chamran University, Tehran, Iran.
Iran J Pharm Res. 2017 Summer;16(3):1185-1189.
AcrAB-TolC is a major efflux pump in . It was reported that is overexpressed and involves in improving the organic solvent tolerance level in mutants that are resistant to several antibiotics, such as ciprofloxacin. Low and intermediate levels resistance did not improve organic solvent tolerance. Thus, it was decided to measure tolC expression and organic solvent tolerance in high level ciprofloxacin resistant mutants. tolC expression was measured by real time PCR and organic solvent tolerance assay was conducted by counting bacterial colonies on LBGMg agar. Results showed that tolC expression was increased significantly (P<0.05) and organic solvent tolerance was slightly improved in high resistant mutants. It was concluded that high organic solvent tolerance may need higher expression of tolC.
AcrAB-TolC是……中的一种主要外排泵。据报道，……在对几种抗生素（如环丙沙星）具有抗性的……突变体中过表达，并参与提高有机溶剂耐受性水平。低水平和中等水平抗性并未提高有机溶剂耐受性。因此，决定在高水平环丙沙星抗性突变体中测量tolC表达和有机溶剂耐受性。通过实时PCR测量tolC表达，并通过计算LBGMg琼脂上的细菌菌落进行有机溶剂耐受性测定。结果表明，高抗性突变体中tolC表达显著增加（P<0.05），有机溶剂耐受性略有提高。得出的结论是，高有机溶剂耐受性可能需要tolC更高的表达。
