伊朗巴博勒医院工作人员鼻腔携带者中分离出的菌株的分子特征分析

Molecular characterization of strains isolated among hospital staff nasal carriers of Babol, Iran.

作者信息

Sabbagh Parisa, Ebrahimzadeh-Namvar Amirmorteza, Ferdosi-Shahandashti Elaheh, Javanian Mostafa, Khafri Soraya, Rajabnia Mehdi

机构信息

Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Department of Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

出版信息

Caspian J Intern Med. 2017 Fall;8(4):311-316. doi: 10.22088/cjim.8.4.311.

DOI:10.22088/cjim.8.4.311

PMID:29201324

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5686312/

Abstract

BACKGROUND

(s. aureus) nasal carriers, particularly the healthcare staff can be considered as a potential source for the spread of resistant strains. The aim of this study was to determine the molecular characterization of S. aureus strains isolated among the staff nasal carriers in one of the teaching hospitals in Babol.

METHODS

A total of 120 nasal swabs were taken from the staff of Ayatollah Rouhani Hospital Babol during 2016. The antibiotic resistance pattern was performed by disc diffusion method for 13 antibiotics, including cefoxitin, cephalothin, teicoplanin, vancomycin, daptomycin, oxacillin, amoxicillin, amikacin, linezolid, ciprofloxacin, levofloxacin, erythromycin, rifampin, according to the CLSI 2015. Polymerase chain reaction (PCR) was used to detect mecA and pvl genes. Finally, the different SCCmec types were determined by multiplex- PCR method.

RESULTS

Among the 120 collected specimens, 40(33.3%) S. aureus isolates were approved. 28(70%) of strains were identified as methicillin-resistant Staphylococcus aureus (MRSA) and the frequency of pvl gene was confirmed 2(5%). Based on the multiplex PCR assay, four different SCCmec types were detected as 35.7% type I, 14.2% type III, 7.1% type II and 3.5% type IV. By a disc diffusion method, no resistance pattern was observed to vancomycin, while 100% of strains were resistant to amoxicillin.

CONCLUSION

Consequently our results illustrated that isolated S. aureus strains among the staff nasal carriers via mentioned molecular characterization may lead to increase the nosocomial persistent infections in hospitalized patients and also health care workers.

摘要

背景

金黄色葡萄球菌鼻腔携带者，尤其是医护人员，可被视为耐药菌株传播的潜在源头。本研究的目的是确定在巴博尔的一家教学医院的医护人员鼻腔携带者中分离出的金黄色葡萄球菌菌株的分子特征。

方法

2016年期间，从巴博尔阿亚图拉·鲁哈尼医院的工作人员中总共采集了120份鼻拭子。根据2015年临床和实验室标准协会（CLSI）的标准，采用纸片扩散法对包括头孢西丁、头孢噻吩、替考拉宁、万古霉素、达托霉素、苯唑西林、阿莫西林、阿米卡星、利奈唑胺、环丙沙星、左氧氟沙星、红霉素、利福平在内的13种抗生素进行耐药模式检测。采用聚合酶链反应（PCR）检测mecA和pvl基因。最后，通过多重PCR方法确定不同的葡萄球菌染色体盒式Mec（SCCmec）类型。

结果

在收集的120份标本中，有40份（33.3%）金黄色葡萄球菌分离株得到确认。28份（70%）菌株被鉴定为耐甲氧西林金黄色葡萄球菌（MRSA），pvl基因的频率经确认是2份（5%）。基于多重PCR分析，检测到四种不同的SCCmec类型，分别为I型35.7%、III型14.2%、II型7.1%和IV型3.5%。通过纸片扩散法，未观察到对万古霉素的耐药模式，而100%的菌株对阿莫西林耐药。