Department of Plastic Surgery and Reconstructive Microsurgery, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Biomed Res Int. 2017;2017:2421585. doi: 10.1155/2017/2421585. Epub 2017 Oct 11.
The aim of our study was to investigate the impact of abdominal wall reconstruction surgery on tissue anatomy and to explore how flap surgery influences the patient's immune status.
Experimental abdominal wall defects were created in 8 (swine) animal models. The animals were divided into two groups: 4 swine were euthanized one month after surgery for the biopsies retrieval purpose and the other 4 swine were kept alive and the collection of blood samples has been done 6 months after surgery. In order to evaluate the relative gene expression in operated-on animal cohorts we compared them with samples from 4 healthy swine used as controls.
The inflammatory process was present in all types of repairs. Collagen I deposition was higher in the flap repairs. The expression level for the genes related to immune response after 6 months from surgery was relatively similar to the control group except minor alteration registered in the case of two swine models.
Our findings indicate a less pronounced proinflammatory response to surgical trauma in animal models after flap surgery. The postoperative levels of the inflammatory cytokines did not show significant differences after abdominal wall reconstruction using flap surgery.
本研究旨在探讨腹壁重建手术对组织解剖结构的影响，并探讨皮瓣手术如何影响患者的免疫状态。
在 8 个（猪）动物模型中创建实验性腹壁缺损。将动物分为两组：4 只猪在手术后一个月因活检目的而被安乐死，另外 4 只猪存活下来，并在手术后 6 个月采集血样。为了评估手术动物队列中的相对基因表达，我们将其与作为对照的 4 只健康猪的样本进行了比较。
所有类型的修复均存在炎症过程。皮瓣修复的 I 型胶原沉积更高。手术后 6 个月与免疫反应相关的基因表达水平与对照组相对相似，除了两个猪模型中记录到的微小改变。
我们的发现表明，在皮瓣手术后的动物模型中，手术创伤的促炎反应程度较轻。使用皮瓣手术进行腹壁重建后，炎症细胞因子的术后水平没有显著差异。
