1Department of Biomedical Sciences,University of Prince Edward Island,Canada.
Acta Neuropsychiatr. 2018 Jun;30(3):181-186. doi: 10.1017/neu.2017.32. Epub 2017 Dec 5.
Stroke patients often suffer from delayed disturbances of mood and cognition. In rodents, the prefrontal cortex (PFC) is involved in both higher order cognition and emotion. Our objective was to determine if bilateral focal ischaemic lesions restricted to the medial prefrontal cortex (mPFC) could be used to model post-stroke anxiety and/or cognitive deficits.
Groups of adult male Sprague-Dawley rats (n=9) received bilateral injections of either endothelin-1 (ET-1) (400 pmol) or vehicle (artificial cerebrospinal fluid) into the mPFC and were tested at various times using both a test of temporal order memory and in an elevated plus maze. Lesions were verified histologically.
ET-1 lesioned rats had reduced mobility on post-surgery day 8 that had resolved by day 29 at which time they spent significantly more time in the closed arm of the plus maze CONCLUSION: We conclude that ischaemic lesions localised to the mPFC can be used to model post-stroke anxiety in rats.
中风患者常伴有情绪和认知障碍。在啮齿类动物中，前额皮质（PFC）参与高级认知和情绪功能。本研究旨在探讨双侧局限于内侧前额皮质（mPFC）的局灶性缺血性损伤是否可用于模拟中风后焦虑和/或认知缺陷。
成年雄性 Sprague-Dawley 大鼠（n=9）接受双侧内皮素-1（ET-1）（400 pmol）或载体（人工脑脊液）注射到 mPFC，并在不同时间使用时间顺序记忆测试和高架十字迷宫进行测试。通过组织学验证损伤。
ET-1 损伤组大鼠在术后第 8 天移动能力下降，第 29 天恢复正常，此时它们在高架十字迷宫的闭臂中花费的时间显著增加。
我们得出结论，局灶性局限于 mPFC 的缺血性损伤可用于模拟大鼠中风后的焦虑。
