Developmental Biology Program, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain.
Elife. 2017 Dec 5;6:e30668. doi: 10.7554/eLife.30668.
During vertebrate heart development, two progenitor populations, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of the heart tube (HT), with the FHF contributing the left ventricle and part of the atria, and the SHF the rest of the heart. Here, we study the dynamics of cardiac differentiation and morphogenesis by tracking individual cells in live analysis of mouse embryos. We report that during an initial phase, FHF precursors differentiate rapidly to form a cardiac crescent, while limited morphogenesis takes place. In a second phase, no differentiation occurs while extensive morphogenesis, including splanchnic mesoderm sliding over the endoderm, results in HT formation. In a third phase, cardiac precursor differentiation resumes and contributes to SHF-derived regions and the dorsal closure of the HT. These results reveal tissue-level coordination between morphogenesis and differentiation during HT formation and provide a new framework to understand heart development.
在脊椎动物心脏发育过程中，两个祖细胞群体，即第一和第二心脏场（FHF，SHF），依次对心管（HT）的纵向细分做出贡献，其中 FHF 贡献左心室和部分心房，而 SHF 贡献心脏的其余部分。在这里，我们通过对活体分析的小鼠胚胎中的单个细胞进行追踪，研究了心脏分化和形态发生的动力学。我们报告说，在初始阶段，FHF 前体迅速分化形成心脏新月形，而形态发生有限。在第二阶段，没有分化发生，而广泛的形态发生，包括内脏中胚层在肠上皮上滑动，导致 HT 形成。在第三阶段，心脏前体细胞的分化恢复，并有助于 SHF 衍生区域和 HT 的背侧闭合。这些结果揭示了 HT 形成过程中形态发生和分化之间的组织水平协调，并为理解心脏发育提供了新的框架。
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