USDA, ARS, National Animal Disease Center, Ames, Iowa, USA
USDA, ARS, National Animal Disease Center, Ames, Iowa, USA.
Infect Immun. 2018 Feb 20;86(3). doi: 10.1128/IAI.00559-17. Print 2018 Mar.
is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes and a wide range of immune evasion capabilities, efficacious vaccines are not readily available. The selection of protein candidates for inclusion in a vaccine was accomplished by identifying fitness genes through a functional genomics screen and selecting conserved predicted surface-associated proteins. Five candidate proteins were selected for evaluation in a vaccine trial and administered both intranasally and intramuscularly with one of two different adjuvant formulations. Clinical protection was evaluated by subsequent intranasal challenge with virulent While subunit vaccination with the proteins induced IgG antibodies to each individual protein and a cellular immune response to the pool of proteins and provided substantial protection from challenge with virulent , the immune response elicited and the degree of protection were dependent on the parenteral adjuvant given. Subunit vaccination induced IgG reactive against different serotypes, indicating a potential for cross protection.
是一种常见的呼吸道细菌，也是猪最重要的侵袭性病原体之一，常引起脑膜炎、关节炎和败血症。由于存在许多血清型和广泛的免疫逃避能力，有效的疫苗并不容易获得。通过功能基因组筛选鉴定适应性基因，并选择保守的预测表面相关蛋白，从而选择用于疫苗的蛋白质候选物。选择了 5 种候选蛋白进行疫苗试验评估，并与两种不同佐剂配方中的一种一起经鼻内和肌肉内给药。通过随后用强毒进行鼻内攻毒来评估临床保护效果。虽然用 蛋白进行亚单位疫苗接种会诱导针对每种蛋白的 IgG 抗体和针对蛋白池的细胞免疫反应，从而为强毒攻毒提供了实质性保护，但免疫反应的强度和保护程度取决于所给的佐剂类型。亚单位疫苗接种诱导针对不同 血清型的 IgG 反应，表明有交叉保护的潜力。
