Giannoulaki E E, Kalpaxis D L, Tentas C, Fessas P
First Department of Internal Medicine, General State Hospital Agios Andreas, Patras, Greece.
Clin Chem. 1989 Mar;35(3):396-9.
Total lactate dehydrogenase (LDH; EC 1.1.1.27) activity and the percentage distribution of LDH isoenzymes were determined in 127 patients with malignant diseases. A shift in the isoenzyme patterns was observed toward the M-type, with an increase in the percentage of LDH-4 and LDH-5 isoenzymes and a slight increase in total LDH activity of all patients. Serum samples from 68 of the patients contained an abnormal isoenzyme of LDH, "LDH-1 ex," that, on agarose gel electrophoresis at pH 8.6, migrated between albumin and LDH-1 isoenzyme. Chemotherapy, radiotherapy, or surgical removal of the tumor was accompanied by disappearance of this abnormal isoenzyme. The heat stability of LDH-1 ex isoenzyme appears to be similar to that of LDH-1 but greater than that of the other LDH isoenzymes. Statistical analysis of these data demonstrated a significant correlation between malignancy and the appearance of LDH-1 ex isoenzyme (P less than 0.001). In contrast, the relationship between LDH-1 ex isoenzyme and metastasis or anatomical location of the malignancy is not statistically important (P less than 0.1).
测定了127例恶性疾病患者的总乳酸脱氢酶（LDH；EC 1.1.1.27）活性及LDH同工酶的百分比分布。观察到同工酶模式向M型转变，所有患者的LDH-4和LDH-5同工酶百分比增加，总LDH活性略有增加。68例患者的血清样本中含有异常的LDH同工酶“LDH-1 ex”，在pH 8.6的琼脂糖凝胶电泳中，其迁移位置在白蛋白和LDH-1同工酶之间。肿瘤的化疗、放疗或手术切除伴随着这种异常同工酶的消失。LDH-1 ex同工酶的热稳定性似乎与LDH-1相似，但高于其他LDH同工酶。对这些数据的统计分析表明，恶性肿瘤与LDH-1 ex同工酶的出现之间存在显著相关性（P<0.001）。相比之下，LDH-1 ex同工酶与转移或恶性肿瘤的解剖位置之间的关系在统计学上并不重要（P<0.1）。
