p16、细胞周期蛋白D1、视网膜母细胞瘤蛋白（Rb）及MIB-1水平在颅底脊索瘤和软骨肉瘤中的临床病理意义
Clinicopathological significance of p16, cyclin D1, Rb and MIB-1 levels in skull base chordoma and chondrosarcoma.
作者信息
Liu Jun-Qi, Zhang Qiu-Hang, Wang Zhen-Lin
机构信息
Xuanwu Hospital Capital Medical University, China.
出版信息
World J Otorhinolaryngol Head Neck Surg. 2015 Oct 24;1(1):50-56. doi: 10.1016/j.wjorl.2015.09.005. eCollection 2015 Sep.
OBJECTIVE
To investigate the expression of p16, cyclin D1, retinoblastoma tumor suppressor protein (Rb) and MIB-1 in skull base chordoma and chondrosarcoma tissues, and to determine the clinicopathological significance of the above indexes in these diseases.
METHODS
A total of 100 skull base chordoma, 30 chondrosarcoma, and 20 normal cartilage tissue samples were analyzed by immunohistochemistry. The expression levels of p16, cyclinD1, Rb and MIB-1 proteins were assessed for potential correlation with the clinicopathological features.
RESULTS
As compared to normal cartilage specimen (control), there was decreased expression of p16, and increased expression of cyclin D1, Rb and MIB-1 proteins, in both skull base chordoma and chondrosarcoma specimens. MIB-1 LI levels were significantly increased in skull base chordoma specimens with negative expression of p16, and positive expression of cyclin D1 and Rb ( < 0.05). Significantly elevated MIB-1 LI was also detected in skull base chondrosarcoma tissues, while there was negative expression of p16, cyclin D1 and Rb ( < 0.05). In skull base chordoma, p16 negatively correlated with cyclin D1 and Rb, while cyclin D1 positively correlated with Rb. Additionally, p16, cyclin D1, Rb, or MIB-1 expression showed no correlation with age, gender, or pathological classification of patients with skull base chordoma ( > 0.05). However, p16 and MIB-1 levels correlated with the intradural invasion, and expression of p16, Rb and MIB-1 correlated with the number of tumor foci ( < 0.05). Further, the expression of p16 and MIB-1 appeared to correlate with the prognosis of patients with skull base chordoma.
CONCLUSIONS
The abnormal expression of p16, cyclin D1 and Rb proteins might be associated with the tumorigenesis of skull base chordoma and chondrosarcoma.
目的
研究p16、细胞周期蛋白D1、视网膜母细胞瘤肿瘤抑制蛋白（Rb）和MIB-1在颅底脊索瘤和软骨肉瘤组织中的表达情况，并确定上述指标在这些疾病中的临床病理意义。
方法
采用免疫组织化学方法分析100例颅底脊索瘤、30例软骨肉瘤及20例正常软骨组织样本。评估p16、细胞周期蛋白D1、Rb和MIB-1蛋白的表达水平与临床病理特征之间的潜在相关性。
结果
与正常软骨标本（对照）相比，颅底脊索瘤和软骨肉瘤标本中p16表达降低，细胞周期蛋白D1、Rb和MIB-1蛋白表达增加。在p16阴性、细胞周期蛋白D1和Rb阳性表达的颅底脊索瘤标本中，MIB-1 LI水平显著升高（P<0.05）。在p16、细胞周期蛋白D1和Rb均阴性表达的颅底软骨肉瘤组织中也检测到MIB-1 LI显著升高（P<0.05）。在颅底脊索瘤中，p16与细胞周期蛋白D1和Rb呈负相关，而细胞周期蛋白D1与Rb呈正相关。此外，p16、细胞周期蛋白D1、Rb或MIB-1的表达与颅底脊索瘤患者的年龄、性别或病理分类无关（P>0.05）。然而，p16和MIB-1水平与硬膜内侵犯相关，p16、Rb和MIB-1的表达与肿瘤灶数量相关（P<0.05）。此外，p16和MIB-1的表达似乎与颅底脊索瘤患者的预后相关。
结论
p16、细胞周期蛋白D1和Rb蛋白的异常表达可能与颅底脊索瘤和软骨肉瘤的发生有关。
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