Rb、p16、Cyclin D1 蛋白在软组织肉瘤中的预后作用。
Prognostic role of Rb, p16, Cyclin D1 proteins in soft tissue sarcomas.
机构信息
Department of Internal Medicine, St. Vincent's Hospital, The Catholic University of Korea School of Medicine, Suwon, Korea.
出版信息
Cancer Res Treat. 2010 Sep;42(3):144-50. doi: 10.4143/crt.2010.42.3.144. Epub 2010 Sep 30.
PURPOSE
The aim of this study was to determine the expressions of Rb, p16, and cyclin D1 in soft tissue sarcomas, and we also wanted to identify the prognostic factors according to the clinicalpathologic features.
MATERIALS AND METHODS
We reviewed the charts and radiographic films of 66 sarcoma patients. Tissue samples were collected from these patients. Immunochemistry was performed using formalin-fixed, paraffin-embedded tissue samples to examine the expressions of p16, Rb, and cyclin D1 proteins.
RESULTS
The median duration of overall survival was 47.8 months (range, 20.0 to 70.7 months) and the 5 years survival rate was 39%. As for the correlation between the degree of immunohistochemical staining for Rb protein and the histological tumor grades, there was a significant difference with a p-value of 0.019. However, no significant correlation was shown for p16 and cyclin D1. The overall survival duration of the Rb negative group (staining cell <20%) and the heterogeneous group (cell staining 20 to 80%) was 53.5±6.6 months and the overall survival duration of the Rb homogeneous group was 18.3±6.4 months, and there was a significant difference with a p-value of 0.016. However, no significant difference was shown between the survival rate according to the p16 and cyclin D1 expressions. On the multivariate analysis that was done with Rb, p16, the tumor size, grade and site, and patient age, the Rb gene expression was the most significant independent prognostic factor with a risk ratio of 3.01 (p=0.04).
CONCLUSION
The expression of Rb protein was correlated with the histologic grade and overall survival of patients with soft tissue sarcomas.
目的
本研究旨在检测软组织肉瘤中 Rb、p16 和 cyclin D1 的表达情况，并根据临床病理特征确定预后因素。
材料与方法
我们回顾了 66 例肉瘤患者的病历和影像学资料。采集这些患者的组织样本，应用免疫组织化学方法检测 p16、Rb 和 cyclin D1 蛋白的表达。
结果
所有患者的中位总生存时间为 47.8 个月（20.0～70.7 个月），5 年生存率为 39%。Rb 蛋白免疫组化染色程度与组织学肿瘤分级之间存在显著相关性（p=0.019），而 p16 和 cyclin D1 与组织学分级之间无相关性。Rb 阴性（染色细胞<20%）和异质性组（染色细胞 20%～80%）的总生存时间为 53.5±6.6 个月，Rb 均质性组为 18.3±6.4 个月，两组之间存在显著差异（p=0.016）。p16 和 cyclin D1 表达与生存率之间无显著差异。在对 Rb、p16、肿瘤大小、分级和部位以及患者年龄进行多因素分析时，Rb 基因表达是最显著的独立预后因素，风险比为 3.01（p=0.04）。
结论
Rb 蛋白的表达与软组织肉瘤的组织学分级和总生存率相关。
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