工程化大肠杆菌中三萜类龙涎香醇的异源生物合成。
Heterologous biosynthesis of triterpenoid ambrein in engineered Escherichia coli.
作者信息
Ke Di, Caiyin Qinggele, Zhao Fanglong, Liu Ting, Lu Wenyu
机构信息
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, People's Republic of China.
Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300350, People's Republic of China.
出版信息
Biotechnol Lett. 2018 Feb;40(2):399-404. doi: 10.1007/s10529-017-2483-2. Epub 2017 Dec 4.
OBJECTIVES
To genetically engineer Escherichia coli for the heterologous biosynthesis of triterpenoid, ambrein, the main bioactive component of ambergris, by constituting a novel squalene-derived ambrein biosynthetic pathway in E. coli.
RESULTS
The ScERG9 gene encoding the squalene synthase (SS) was integrated into the E. coli genome to generate a squalene-producing strain that supplied the central precursor squalene for the formation of cyclic triterpenoids. The mutated squalene-hopene synthase (D377C SHC) and the tetraprenyl-β-curcumene cyclase (BmeTC) were co-expressed with SS to construct a novel ambrein biosynthetic pathway in E. coli. Ambrein was produced at 2.6 mg l.
CONCLUSIONS
An E. coli chassis for ambrein production was constructed by combining the squalene synthesis module with the downstream cyclization module.
目的
通过在大肠杆菌中构建一条新的源自角鲨烯的龙涎香醇生物合成途径，对大肠杆菌进行基因工程改造，以实现龙涎香醇（龙涎香的主要生物活性成分）的异源生物合成。
结果
将编码角鲨烯合酶（SS）的ScERG9基因整合到大肠杆菌基因组中，以产生一个生产角鲨烯的菌株，该菌株为环状三萜类化合物的形成提供中心前体角鲨烯。将突变的角鲨烯-藿烯合酶（D377C SHC）和四异戊烯基-β-姜黄烯环化酶（BmeTC）与SS共表达，在大肠杆菌中构建了一条新的龙涎香醇生物合成途径。龙涎香醇的产量为2.6毫克/升。
结论
通过将角鲨烯合成模块与下游环化模块相结合，构建了一个用于生产龙涎香醇的大肠杆菌底盘。
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