Department of Toxicology, International Institute of Biotechnology and Toxicology (IIBAT), Padappai 601301, Tamil Nadu, India.
Toxicol In Vitro. 2018 Mar;47:165-177. doi: 10.1016/j.tiv.2017.11.010. Epub 2017 Dec 5.
Pyrethroid-mediated changes on microelectrode array (MEA) parameters, such as mean firing rate (MFR), mean burst rate (MBR), and number of active channels (nAC) were investigated by exposing neuronal networks to cumulative concentrations (0.01, 0.1, 1, 10, and 100μM) of pyrethroids (Type-1, bifenthrin and permethrin; Type-2, beta-cyfluthrin, cypermethrin, deltamethrin, and lambda-cyhalothrin). The average MFR, MBR, and nAC increased for all pyrethroids (except nAC for deltamethrin) at lower concentrations and decreased at higher concentrations. The increase in the average MFR, MBR, and nAC was not statistically significant in most of the cases. Increase in MFR, MBR, and nAC was observed in 19/19, 18/19, and 12/19 individual experiments, respectively, at lower concentrations. The ICs calculated on MEA parameters were more or less similar. The relative potencies calculated on the ICs of MEA parameters had a strong positive correlation. These observations indicate that the MEA parameters MFR, MBR, and nAC follow the same trend for pyrethroid-mediated changes, and provide a similar outcome. The rank orders of relative potencies on the ICs of the MEA parameters distinguish type-1 pyrethroids from type-2 pyrethroids, with type-2 being more potent. As increase in MFR at the lower concentrations of pyrethroids was observed in all the individual experiments (19/19), it may be considered as the characteristic effect of pyrethroids on neuronal excitability.
拟除虫菊酯对微电极阵列（MEA）参数的影响，如平均放电率（MFR）、平均爆发率（MBR）和活跃通道数（nAC），通过将神经元网络暴露于拟除虫菊酯（1 型，联苯菊酯和氯菊酯；2 型，β-氯氟氰菊酯、氯氰菊酯、溴氰菊酯和氯氟氰菊酯）的累积浓度（0.01、0.1、1、10 和 100μM）来进行研究。所有拟除虫菊酯（除溴氰菊酯的 nAC 外）在较低浓度下都会增加平均 MFR、MBR 和 nAC，而在较高浓度下则会降低。在大多数情况下，平均 MFR、MBR 和 nAC 的增加并不显著。在较低浓度下，分别有 19/19、18/19 和 12/19 个个体实验观察到 MFR、MBR 和 nAC 的增加。在 MEA 参数上计算出的 IC 或多或少相似。MEA 参数 IC 上计算出的相对效力具有很强的正相关性。这些观察结果表明，MEA 参数 MFR、MBR 和 nAC 对拟除虫菊酯介导的变化遵循相同的趋势，并提供了类似的结果。MEA 参数 IC 上相对效力的秩次能够区分 1 型和 2 型拟除虫菊酯，其中 2 型更有效。由于在所有个体实验中（19/19）都观察到拟除虫菊酯在较低浓度下增加了 MFR，因此可以将其视为拟除虫菊酯对神经元兴奋性的特征性影响。
