Katsumoto F, Miyazaki K, Nakayama F
Kyushu University, Faculty of Medicine, Department of Surgery 1, Fukuoka, Japan.
Hepatology. 1989 Mar;9(3):405-10. doi: 10.1002/hep.1840090310.
The role of Kupffer cells during reparative regeneration of rat liver was investigated with an in vitro experimental model. Conditioned media from primary cultures of Kupffer cells isolated from intact and regenerating liver were added to primary cultures of hepatocytes, and [3H]thymidine incorporation into DNA was studied. Kupffer cell-conditioned media from intact liver and regenerating remnant liver significantly stimulated DNA synthesis in hepatocytes as compared with control media (p less than 0.05). Moreover, the stimulating activity of Kupffer cells prepared from regenerating liver at 6 and 12 hr after partial hepatectomy was significantly higher than that of Kupffer cells from untreated rats (p less than 0.05). The activity was found in serum-free conditioned media. This stimulating activity exponentially increased as the increase of the number of the cultured cells, indicating that the stimulating activity was released directly by cultured Kupffer cells. These results suggest that Kupffer cells stimulate DNA synthesis in hepatocytes by producing and releasing certain factor(s) at an early stage of liver regeneration after partial hepatectomy.
利用体外实验模型研究了库普弗细胞在大鼠肝脏修复性再生过程中的作用。将从完整肝脏和再生肝脏分离的库普弗细胞原代培养的条件培养基添加到肝细胞原代培养物中，并研究了[3H]胸腺嘧啶核苷掺入DNA的情况。与对照培养基相比，来自完整肝脏和再生残余肝脏的库普弗细胞条件培养基显著刺激了肝细胞中的DNA合成（p<0.05）。此外，部分肝切除术后6小时和12小时从再生肝脏制备的库普弗细胞的刺激活性明显高于未处理大鼠的库普弗细胞（p<0.05）。这种活性存在于无血清条件培养基中。随着培养细胞数量的增加，这种刺激活性呈指数增加，表明刺激活性是由培养的库普弗细胞直接释放的。这些结果表明，库普弗细胞在部分肝切除术后肝脏再生的早期通过产生和释放某些因子来刺激肝细胞中的DNA合成。
