Stenström F, la Cour Jansen J
VA-Ingenjörerna AB, Veolia Water Technologies, Trädgårdsgatan 12, SE-702 12 Örebro, Sweden E-mail:
Water and Environmental Engineering, Department of Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
Water Sci Technol. 2017 Dec;76(11-12):3079-3085. doi: 10.2166/wst.2017.480.
Nitrifiers are the slowest growing bacteria used in conventional biological wastewater treatment. Furthermore, their growth rate is seriously hampered by low temperature. As a result, the volume needed for nitrification dominates the volume of the biological reactors at a wastewater treatment plant. As a way of enhancing nitrification and reducing this volume, bioaugmentation can be used. Nitrifiers from a side-stream plant can be inoculated to the mainstream process, which is thereby boosted. The effect of bioaugmentation can be measured in different ways. This full-scale study focuses on the effect of bioaugmentation from a microbial point of view by using 16S rRNA amplicon sequencing. The study reveals how bioaugmentation increases the diversity of nitrifiers in the mainstream process and in the side-stream plant; that the abundance of nitrifiers is increased in the mainstream process; the interaction between nitrifiers from the side-stream plant and mainstream process; and the effect of bioaugmentation on nitrifying genera and species over time. To our knowledge, this detailed microbial information on nitrifying species during a full-scale bioaugmentation study has not been presented before.
硝化细菌是传统生物废水处理中生长最慢的细菌。此外，它们的生长速率受到低温的严重抑制。因此，硝化所需的体积在废水处理厂的生物反应器体积中占主导地位。作为一种增强硝化作用并减小该体积的方法，可以使用生物强化。来自旁流处理厂的硝化细菌可以接种到主流工艺中，从而提高主流工艺的效率。生物强化的效果可以通过不同方式来衡量。这项全尺寸研究通过使用16S rRNA扩增子测序，从微生物角度关注生物强化的效果。该研究揭示了生物强化如何增加主流工艺和旁流处理厂中硝化细菌的多样性；主流工艺中硝化细菌的丰度如何增加；旁流处理厂的硝化细菌与主流工艺之间的相互作用；以及生物强化随着时间推移对硝化菌属和菌种的影响。据我们所知，此前在全尺寸生物强化研究中尚未呈现过关于硝化菌种的如此详细的微生物信息。
