State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , Changchun, Jilin 130012, China.
Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
Biomacromolecules. 2018 Jan 8;19(1):201-208. doi: 10.1021/acs.biomac.7b01469. Epub 2017 Dec 18.
Conventional photodynamic therapy is severely constrained by the limited light-penetration depth in tissue. Here, we show efficient photodynamic therapy (PDT) mediated by bioluminescence resonance energy transfer (BRET) that overcomes the light-penetration limitation. The photosensitizer Rose Bengal (RB) was loaded in biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles, which were then conjugated with firefly luciferase. Spectroscopic characterizations indicated that BRET effectively activated RB to generate reactive oxygen species (ROS). In vitro studies of the cellular cytotoxicity and photodynamic effect indicated that cancer cells were effectively destroyed by BRET-PDT treatment. In vivo studies in a tumor-bearing mouse model demonstrated that tumor growth was significantly inhibited by BRET-PDT in the absence of external light irradiation. The BRET-mediated phototherapy provides a promising approach to overcome the light-penetration limitation in photodynamic treatment of deep-seated tumors.
传统的光动力疗法受到组织中有限的光穿透深度的严重限制。在这里，我们展示了通过生物发光共振能量转移（BRET）介导的高效光动力疗法（PDT），克服了光穿透限制。光敏剂 Rose Bengal（RB）被装载在可生物降解的聚（乳酸-共-乙醇酸）（PLGA）纳米颗粒中，然后与萤火虫荧光素酶缀合。光谱特性表明 BRET 有效地激活 RB 以产生活性氧（ROS）。细胞毒性和光动力效应的体外研究表明，BRET-PDT 处理可有效破坏癌细胞。在荷瘤小鼠模型中的体内研究表明，在没有外部光照射的情况下，BRET-PDT 显著抑制了肿瘤生长。BRET 介导的光疗为克服深层肿瘤光动力治疗中的光穿透限制提供了一种有前途的方法。
