Immune Enhancement Effect of Fatty Acids through NF-κB and MAPK Pathways on RAW 264.7 Cells.

is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of on murine macrophages (RAW 264.7 cells). fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. fatty acids also enhanced the expression of critical immune-associated genes, including , and , as well as . Western blotting showed that fatty acids stimulated the NF-κB and MAPK pathways by phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK. fatty acids from different tissues resulted in different levels of NF-κB and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how fatty acids boost immunity in a physiological system, as a potential functional material.