Li Pingchuan, Biligetu Bill, Coulman Bruce E, Schellenberg Michael, Fu Yong-Bi
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5A8.
Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, Swift Current, Saskatchewan, Canada S9H 3X2.
Data Brief. 2017 Sep 22;15:401-406. doi: 10.1016/j.dib.2017.09.030. eCollection 2017 Dec.
Crested wheatgrass [ L. (Gaertn.)] is an important cool-season forage grass widely used for early spring grazing. However, the genomic resources for this non-model plant are still lacking. Our goal was to generate the first set of next generation sequencing data using the genotyping-by-sequencing technique. A total of 272 crested wheatgrass plants representing seven breeding lines, five cultivars and five geographically diverse accessions were sequenced with an Illumina MiSeq instrument. These sequence datasets were processed using different bioinformatics tools to generate contigs for diploid and tetraploid plants and SNPs for diploid plants. Together, these genomic resources form a fundamental basis for genomic studies of crested wheatgrass and other wheatgrass species. The raw reads were deposited into Sequence Read Archive (SRA) database under NCBI accession SRP115373 (https://www.ncbi.nlm.nih.gov/sra?term=SRP115373) and the supplementary datasets are accessible in Figshare (10.6084/m9.figshare.5345092).
扁穗冰草[L. (Gaertn.)]是一种重要的冷季型饲草，广泛用于早春放牧。然而，这种非模式植物的基因组资源仍然匮乏。我们的目标是使用简化基因组测序技术生成第一组二代测序数据。使用Illumina MiSeq仪器对代表7个育种系、5个品种和5个地理来源各异的种质的总共272株扁穗冰草植株进行了测序。这些序列数据集使用不同的生物信息学工具进行处理，以生成二倍体和四倍体植株的重叠群以及二倍体植株的单核苷酸多态性。这些基因组资源共同构成了扁穗冰草和其他冰草物种基因组研究的基础。原始读数已存入NCBI序列读取存档（SRA）数据库，登录号为SRP115373（https://www.ncbi.nlm.nih.gov/sra?term=SRP115373），补充数据集可在Figshare上获取（10.6084/m9.figshare.5345092）。
