Donnerer Josef, Liebmann Ingrid
Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Universitätsplatz 4, A-8010 Graz, Austria.
Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Universitätsplatz 4, A-8010 Graz, Austria.
Neurosci Lett. 2018 Feb 5;665:152-155. doi: 10.1016/j.neulet.2017.12.008. Epub 2017 Dec 5.
The aim of the present study was to investigate the protein levels of BDNF and IL-1ß in the lumbar dorsal horn of the rat by Western blot analysis following a noxious thermal hind paw stimulation. Ten min, 1h and 3h after the combined chemical and thermal stimulation an up to 2-fold increase in BDNF and Il-1ß protein expression was observed in the lumbar dorsal spinal cord. A pretreatment with the opioid analgesic morphine or the glial cell activation inhibitor minocycline partly attenuated protein expression. The present findings indicate that the BDNF and IL-1ß induction within the dorsal horn may be linked to the development of hyperalgesia, and that opioid analgesics and probably inhibitors of glial cell activation can prevent sensitization in the pain pathway at spinal level.
本研究的目的是通过蛋白质印迹分析，在有害热刺激后大鼠腰段背角中研究脑源性神经营养因子（BDNF）和白细胞介素-1β（IL-1β）的蛋白质水平。在化学和热刺激联合作用后10分钟、1小时和3小时，在腰段背侧脊髓中观察到BDNF和IL-1β蛋白表达增加了两倍。用阿片类镇痛药吗啡或胶质细胞激活抑制剂米诺环素预处理可部分减弱蛋白表达。本研究结果表明，背角内BDNF和IL-1β的诱导可能与痛觉过敏的发展有关，并且阿片类镇痛药以及可能的胶质细胞激活抑制剂可以在脊髓水平预防疼痛通路中的致敏作用。
