Spiegel E, Howard L, Spiegel M
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.
J Morphol. 1989 Jan;199(1):71-92. doi: 10.1002/jmor.1051990107.
The extracellular matrix surrounding the sea urchin embryo (outer ECM) contains fibers and granules of various sizes which are organized in recognizable patterns as shown by ultrastructural studies, particularly stereoimaging techniques. The use of the ruthenium red method for retaining and staining the ECM, with modifications of the Luft (Anatomical Record 171:347-368, 1971) method for invertebrate embryos, allows for the clarification of certain structures, particularly fiber compaction in the interzonal region, and microvillus-associated bodies. The inner ECM in the sea urchin embryo includes the basal lamina and blastocoel matrix. Stereoimages show that the fibers which are loosely distributed in the blastocoel matrix become compacted around the periphery of the blastocoel to form the basal lamina. The ruthenium red method was also used on a number of marine invertebrate embryos and larvae, representing different phyla, to facilitate comparisons between their surface coats. The similarities observed in the specimens shown suggest that ECMs are widely found on marine invertebrate eggs, embryos, and larvae, and that they resemble vertebrate ECMs and may, therefore, have similar functions.
海胆胚胎周围的细胞外基质（外部细胞外基质）包含各种大小的纤维和颗粒，如超微结构研究，特别是立体成像技术所示，它们以可识别的模式组织。使用钌红法保留和染色细胞外基质，并对用于无脊椎动物胚胎的卢夫特方法（《解剖学记录》171:347 - 368，1971年）进行修改，有助于阐明某些结构，特别是中间区域的纤维压实以及微绒毛相关体。海胆胚胎中的内部细胞外基质包括基膜和囊胚腔基质。立体图像显示，松散分布在囊胚腔基质中的纤维在囊胚腔周边压实形成基膜。钌红法还用于许多代表不同门的海洋无脊椎动物胚胎和幼虫，以促进它们表面涂层之间的比较。所示标本中观察到的相似性表明，细胞外基质广泛存在于海洋无脊椎动物的卵、胚胎和幼虫上，并且它们类似于脊椎动物的细胞外基质，因此可能具有相似的功能。
