发酵初榨椰子油的血管生成和伤口愈合能力： 以及 研究。 （你提供的原文中“and”前后内容不完整，可能影响准确理解，以上是按现有内容翻译）
Angiogenic and wound healing potency of fermented virgin coconut oil: and studies.
作者信息
Ibrahim Ahmad H, Li Haibo, Al-Rawi Sawsan S, Majid Aman Shah Abdul, Al-Habib Omar Am, Xia Xiaobo, Majid Amin Ms Abdul, Ji Dan
机构信息
Depatment of Biology, Faculty of Sciences, University of ZakhoKurdistan Region, Iraq.
Department of Ophthalmology, Xiangya Hospital, Central South UniversityChangsha 410000, Hunan Province, China.
出版信息
Am J Transl Res. 2017 Nov 15;9(11):4936-4944. eCollection 2017.
OBJECTIVE
The process of wound healing involves activation of keratinocytes, fibroblasts, endothelial cells, etc. Angiogenesis is crucial during the process of wound healing. Virgin coconut oil is widely utilized in South Asia for various purposes including food, medicinal and industrial applications. This study aimed to evaluate the potency of fermented virgin coconut oil (FVCO) in angiogenesis and wound healing via both and assays.
METHODS
Human umbilical vein endothelial (HUVEC), fibroblast (CCD-18) and retinal ganglion (RGC-5) cells were cultured in medium containing different concentrations of FVCO. The proliferation, migration and morphological changes of cells were determined. The angiogenic effect of FVCO was evaluated by rat aortic assay. The therapeutic effect of FVCO on wound healing was further assessed in a wound excision model in Sprague Dawley rats. The expression of phospho-VEGFR2 (vascular endothelial growth factor receptor 2) in HUVECs was detected by Western blot.
RESULTS
FVCO (6 and 12 µg/mL) significantly improved the proliferation of HUVEC, CCD-18 and RGC-5 cells ( < 0.05 or 0.01). FVCO (25 µg/mL) markedly increased the migration ability of CCD-18 and RGC-5 cells ( < 0.05). FVCO did not affect cell morphology as indicated by fluorescein diacetate (FDA), rhodamine 123 and Hoechst staining. FVCO (25, 50 and 100 µg/mL) significantly stimulated the blood vessel formation as compared with negative control ( < 0.05). Rats in FVCO group had significantly smaller wound size, higher wound healing percentage, and shorter wound closure time when compared with control group since day 8 ( < 0.05), suggesting that oral FVCO administration notably promoted the wound healing process. FVCO treatment (6 and 12 µg/mL) significantly enhanced the phospho-VEGFR2 expression in HUVECs ( = 0.006 and 0.000, respectively).
CONCLUSION
Our study confirms a high angiogenic and wound healing potency of FVCO that might be mediated by the regulation of VEGF signing pathway.
目的
伤口愈合过程涉及角质形成细胞、成纤维细胞、内皮细胞等的激活。血管生成在伤口愈合过程中至关重要。初榨椰子油在南亚被广泛用于各种用途，包括食品、医药和工业应用。本研究旨在通过体内和体外实验评估发酵初榨椰子油（FVCO）在血管生成和伤口愈合方面的效力。
方法
将人脐静脉内皮细胞（HUVEC）、成纤维细胞（CCD - 18）和视网膜神经节细胞（RGC - 5）培养在含有不同浓度FVCO的培养基中。测定细胞的增殖、迁移和形态变化。通过大鼠主动脉实验评估FVCO的血管生成作用。在Sprague Dawley大鼠的伤口切除模型中进一步评估FVCO对伤口愈合的治疗效果。通过蛋白质免疫印迹法检测HUVECs中磷酸化VEGFR2（血管内皮生长因子受体2）的表达。
结果
FVCO（6和12μg/mL）显著提高了HUVEC、CCD - 18和RGC - 5细胞的增殖（P < 0.05或0.01）。FVCO（25μg/mL）显著提高了CCD - 18和RGC - 5细胞的迁移能力（P < 0.05）。荧光素二乙酸酯（FDA）、罗丹明123和Hoechst染色表明FVCO不影响细胞形态。与阴性对照相比，FVCO（25、50和100μg/mL）显著刺激血管生成（P < 0.05）。自第8天起，与对照组相比，FVCO组大鼠的伤口尺寸显著更小，伤口愈合百分比更高，伤口闭合时间更短（P < 0.05），表明口服FVCO显著促进了伤口愈合过程。FVCO处理（6和12μg/mL）显著增强了HUVECs中磷酸化VEGFR2的表达（分别为P = 0.006和0.000）。
结论
我们的研究证实FVCO具有较高的血管生成和伤口愈合效力，这可能是由VEGF信号通路的调节介导的。
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