Center for Proteomics and Bioinformatics and Department of Nutrition, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH, 44106-4988, USA.
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
Adv Exp Med Biol. 2017;1009:229-238. doi: 10.1007/978-981-10-6038-0_14.
Integrative structure modeling is an emerging method for structural determination of protein-protein complexes that are challenging for conventional structural techniques. Here, we provide a practical protocol for implementing our integrated iSPOT platform by integrating three different biophysical techniques: small-angle X-ray scattering (SAXS), hydroxyl radical footprinting, and computational docking simulations. Specifically, individual techniques are described from experimental and/or computational perspectives, and complementary structural information from these different techniques are integrated for accurate characterization of the structures of large protein-protein complexes.
整合结构建模是一种新兴的方法，可用于确定常规结构技术具有挑战性的蛋白质-蛋白质复合物的结构。在这里，我们通过整合三种不同的生物物理技术：小角度 X 射线散射（SAXS）、羟基自由基足迹分析和计算对接模拟，提供了实现我们的整合 iSPOT 平台的实用方案。具体来说，从实验和/或计算的角度描述了各个技术，并整合了这些不同技术的互补结构信息，以准确表征大型蛋白质-蛋白质复合物的结构。
