Wang C Z, Wen S Q, Shi M S, Yu X E, Wang X J, Pan Y L, Zhang Y F, Li H, Tan J Z
MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China.
Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, China University of Political Science and Law, Beijing 100088, China.
Fa Yi Xue Za Zhi. 2017 Aug;33(4):357-362. doi: 10.3969/j.issn.1004-5619.2017.04.005. Epub 2017 Aug 25.
To identify the Y-chromosomal genetic types for the soldier's remains from Huaihai Campaign, and to offer a clue for search of their paternal relatives.
DNA of the remains were extracted by the ancient DNA extraction method. Yfiler kit was used for the multiplex amplification of 17 Y-STR loci. The haplogroups of the samples were speculated. Detailed genotyping of the selected Y-SNP was performed based on the latest Y-chromosome phylogenetic tree. Haplotype-sharing analysis was done based on the data of Y-SNP and Y-STR, the closest modern individual information to the genetic relationship of remains was gained.
A total of 8 Y-STR haplotypes were observed on 17 Y-STR loci of 8 male individuals. Furthermore, 6 Y-SNP haplogroups were identified, which were O2a1-M95+, O1a1-P203+, O3*-M122+/M234-, D1-M15+, C3*-ST and R1a1-M17+.
Identification of Y-chromosomal genetic types for the soldier's remains from Huaihai Campaign shows a reference value on inferring the geographical origins of old materials.
确定淮海战役牺牲战士遗骸的Y染色体遗传类型，为寻找其 paternal 亲属提供线索。
采用古DNA提取方法提取遗骸DNA。使用Yfiler试剂盒对17个Y-STR基因座进行多重扩增。推测样本的单倍群。根据最新的Y染色体系统发育树对选定的Y-SNP进行详细基因分型。基于Y-SNP和Y-STR数据进行单倍型共享分析，获得与遗骸遗传关系最接近的现代个体信息。
在8名男性个体的17个Y-STR基因座上共观察到8种Y-STR单倍型。此外，鉴定出6种Y-SNP单倍群，分别为O2a1-M95+、O1a1-P203+、O3*-M122+/M234-、D1-M15+、C3*-ST和R1a1-M17+。
淮海战役牺牲战士遗骸Y染色体遗传类型的鉴定对推断旧材料的地理来源具有参考价值。
