Winet H
Department of Orthopaedics, University of Southern California, Los Angeles.
Microvasc Res. 1989 Jan;37(1):105-14. doi: 10.1016/0026-2862(89)90075-7.
A horizontal intravital microscope has been built which utilizes the telescope optics format with infinity-corrected objectives. The intravital stage accomodates a rabbit into which a tibial bone chamber has been implanted. The chamber is itself an optical component and is considered an integral part of the system. Each animal is observed in supine position with its implanted leg held in an adjustable spring-loaded yoke which aligns the bone chamber in the optical axis. Long working-distance objectives and condensers allow for viewing of the slit-gap tissue in the bone chamber with both transmitted and epi-illumination; the latter being applied for fluorescence studies. A unique feature of the system is a transfer lens holder which can be relocated within the microscope tube. This adjustable lens holder allows for changing of transfer lenses, thereby freeing the investigator to utilize objective lenses with preferred numerical apertures for a given image size.
已构建了一台卧式活体显微镜，它采用了带有无限远校正物镜的望远光学系统形式。活体载物台可容纳一只已植入胫骨骨腔的兔子。该骨腔本身就是一个光学部件，被视为系统的一个组成部分。每只动物均仰卧观察，其植入腿被固定在一个可调节的弹簧加载轭架中，该轭架将骨腔对准光轴。长工作距离物镜和聚光镜允许通过透射光和落射光观察骨腔内的狭缝间隙组织；落射光用于荧光研究。该系统的一个独特之处是一个可在显微镜镜筒内重新定位的转接透镜架。这个可调节的透镜架允许更换转接透镜，从而使研究人员能够在给定图像尺寸下自由使用具有首选数值孔径的物镜。
