眼部弓形虫病中CCR5趋化因子受体基因多态性
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis.
作者信息
de Faria Junior Geraldo M, Ayo Christiane M, de Oliveira Amanda P, Lopes Alessandro G, Frederico Fábio B, Silveira-Carvalho Aparecida P, Previato Mariana, Barbosa Amanda P, Murata Fernando H A, de Almeida Junior Gildásio Castello, Siqueira Rubens Camargo, de Mattos Luiz C, Brandão de Mattos Cinara C
机构信息
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
出版信息
Acta Trop. 2018 Feb;178:276-280. doi: 10.1016/j.actatropica.2017.12.012. Epub 2017 Dec 6.
CC chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as 'with ocular toxoplasmosis' (G1: n=160) or 'without ocular toxoplasmosis' (G2: n=160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD±19.3, median: 46 [range: 18-95]; G2: mean age: 61.3, SD±13.7, median: 61 [range: 21-87]; G3: mean age: 38.8, SD±17.9, median: 34 [range: 18-80]) was statistically significant (G1 vs.G2: p-value <0.0001; t=7.21; DF=318; G1 vs.G3: p-value <0.0001; t=4.32; DF=318; G2 vs. G3: p-value <0.0001; t=9.62; DF=318). The Nagelkerke r value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value=0.008; OR=0.261), AA (p-value=0.007; OR=2.974) and AG genotypes (p-value=0.018; OR=2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue.
C-C趋化因子受体5（CCR5）是一种趋化因子受体，可影响对感染性疾病和寄生虫病的免疫反应。本研究旨在确定CCR5Δ32和CCR5 59029 A/G基因多态性是否与人类眼弓形虫病的发生有关。对弓形虫血清学检测呈阳性的患者进行分析，并分为“患有眼弓形虫病”组（G1：n = 160）或“未患眼弓形虫病”组（G2：n = 160）。对照组（G3）由160名血清学检测呈阴性的个体组成。CCR5Δ32和CCR5 59029 A/G基因多态性分别通过聚合酶链反应（PCR）和聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）进行鉴定。各组之间的平均年龄存在统计学显著差异（G1：平均年龄：47.3，标准差±19.3，中位数：46 [范围：18 - 95]；G2：平均年龄：61.3，标准差±13.7，中位数：61 [范围：21 - 87]；G3：平均年龄：38.8，标准差±17.9，中位数：34 [范围：18 - 80]）（G1与G2比较：p值<0.0001；t = 7.21；自由度 = 318；G1与G3比较：p值<0.0001；t = 4.32；自由度 = 318；G2与G3比较：p值<0.0001；t = 9.62；自由度 = 318）。Nagelkerke r值为0.040。G1和G2之间在CCR5/CCR5（p值 = 0.008；比值比 = 0.261）、AA（p值 = 0.007；比值比 = 2.974）和AG基因型（p值 = 0.018；比值比 = 2.447）方面存在统计学显著差异。具有CCR5/CCR5基因型且同时具有CCR5 - 59029 AA或AG基因型的个体发生眼弓形虫病的风险更高（高4%），这可能与眼组织中强烈且持续的炎症反应有关。
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