Shih Yo-Seng, Fu Earl, Fu Martin M, Lin Fu-Gong, Chiu Hsien-Chung, Shen E-Chin, Chiang Cheng-Yang
Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, ROC.
J Periodontol. 2014 Nov;85(11):1596-602. doi: 10.1902/jop.2014.130651. Epub 2014 Aug 14.
It has been suggested that genetic factors may predispose individuals to periodontal diseases. The present case-control study aims to test whether the -403 single nucleotide polymorphism of chemokine ligand 5 (CCL5-403) and the 32-bp deletion of CCR5 (CCR5Δ32) polymorphisms are associated with susceptibility to chronic and aggressive periodontitis.
Taiwanese participants (N = 213) were grouped into control group (CG), generalized aggressive periodontitis (GAgP), or chronic periodontitis (CP) groups. DNA samples were obtained from peripheral blood. CCL5-403, evaluated by polymerase chain reaction-restriction fragment length polymorphism, and CCR5Δ32, evaluated by polymerase chain reaction, were compared among the three groups.
There was a significant association between type of periodontitis and having allele A or G in the CCL5-403 polymorphism. GAgP patients were 3.7 times more likely than CP patients and 2.0 times more likely than CG patients to have allele A, instead of allele G, in CCL5-403. GAgP patients were 3.1 times more likely than CG patients to have AG versus GG genotype. GAgP patients were also 5.0 and 19.8 times more likely than CP patients to have AG and AA genotypes, respectively, compared to GG. For the CCR5Δ32 polymorphism, no association was found between the type of periodontitis and having different genotype or allele distributions among GAgP, CP, or CG patients.
The single nucleotide polymorphism of CCL5-403 G substitution by A may play a role in AgP; however, the CCR5Δ32 polymorphism may not.
有研究表明遗传因素可能使个体易患牙周疾病。本病例对照研究旨在检测趋化因子配体5（CCL5 - 403）的 - 403单核苷酸多态性以及CCR5的32碱基对缺失（CCR5Δ32）多态性是否与慢性和侵袭性牙周炎的易感性相关。
将台湾参与者（N = 213）分为对照组（CG）、广泛型侵袭性牙周炎（GAgP）组或慢性牙周炎（CP）组。从外周血获取DNA样本。通过聚合酶链反应 - 限制性片段长度多态性评估CCL5 - 403，通过聚合酶链反应评估CCR5Δ32，并在三组之间进行比较。
牙周炎类型与CCL5 - 403多态性中存在等位基因A或G之间存在显著关联。在CCL5 - 403中，GAgP患者具有等位基因A而非等位基因G的可能性是CP患者的3.7倍，是CG患者的2.0倍。与GG基因型相比，GAgP患者具有AG基因型的可能性是CG患者的3.1倍。与GG相比，GAgP患者具有AG和AA基因型的可能性分别是CP患者的5.0倍和19.8倍。对于CCR5Δ32多态性，在GAgP、CP或CG患者中，未发现牙周炎类型与不同基因型或等位基因分布之间存在关联。
CCL5 - 403由G替换为A的单核苷酸多态性可能在侵袭性牙周炎中起作用；然而，CCR5Δ32多态性可能并非如此。
