啮齿动物皮质、海马体和纹状体神经元的培养。
Culture of Rodent Cortical, Hippocampal, and Striatal Neurons.
作者信息
Facci Laura, Skaper Stephen D
机构信息
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy.
出版信息
Methods Mol Biol. 2018;1727:39-47. doi: 10.1007/978-1-4939-7571-6_3.
Neurons cultured from rodent central nervous system tissues represent important tools in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical, hippocampal, and striatal neuron cell cultures, using either embryonic or postnatal tissue with enzymatic digestion.
从啮齿动物中枢神经系统组织培养的神经元是研究神经退行性疾病机制和神经再生过程的重要工具，包括神经营养因子的促存活和促轴突生长特性。本章介绍了一种详细的方案，用于使用胚胎或出生后组织通过酶消化制备大鼠和小鼠的皮质、海马和纹状体神经元细胞培养物。
Zotero 插件