Gorokhov V V, Knox P P, Korvatovskiy B N, Seifullina N Kh, Goryachev S N, Paschenko V Z
Lomonosov Moscow State University, Faculty of Biology, Moscow, 119991, Russia.
Biochemistry (Mosc). 2017 Nov;82(11):1269-1275. doi: 10.1134/S0006297917110049.
The temperature dependences of tryptophan fluorescence decay kinetics in aqueous glycerol and 1 M trehalose solutions were examined. The fluorescence decay kinetics were recorded in the spectral region of 292.5-417.5 nm with nanosecond time resolution. The kinetics curves were approximated by the sum of three exponential terms, and the spectral distribution (DAS) of these components was determined. An antisymbatic course of fluorescence decay times of two (fast and medium) components in the temperature range from -60 to +10°C was observed. The third (slow) component showed only slight temperature dependence. The antisymbatic behavior of fluorescence lifetimes of the fast and medium components was explained on the assumption that some of the excited tryptophan molecules are transferred from a short-wavelength B-form with short fluorescence lifetime to a long-wavelength R-form with an intermediate fluorescence lifetime. This transfer occurred in the indicated temperature range.
研究了色氨酸在甘油水溶液和1 M海藻糖溶液中的荧光衰减动力学的温度依赖性。在292.5 - 417.5 nm光谱区域内以纳秒时间分辨率记录荧光衰减动力学。动力学曲线用三个指数项之和进行拟合，并确定这些组分的光谱分布（DAS）。在-60至+10°C温度范围内观察到两个（快速和中等）组分的荧光衰减时间呈反对称变化。第三个（慢速）组分仅表现出轻微的温度依赖性。快速和中等组分荧光寿命的反对称行为是基于这样的假设来解释的：一些激发态色氨酸分子从具有短荧光寿命的短波长B型转移到具有中等荧光寿命的长波长R型。这种转移发生在所示的温度范围内。
