ICBAS - Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Future Microbiol. 2018 Jan;13:27-36. doi: 10.2217/fmb-2017-0107. Epub 2017 Dec 11.
The goal of this study was to determine the effectiveness of antibiotics on Staphylococcus epidermidis biofilms with different proportions of dormant bacteria, using clinical and commensal isolates.
MATERIALS & METHODS: The ability of S. epidermidis isolates to develop a dormant state was determined. The susceptibility of biofilms with prevented or induced dormancy to antibiotics was evaluated by enumeration of viable and cultivable cells, and confocal microscopy.
Dormancy was observed in the majority of tested strains. Tetracycline and rifampicin enhanced the development of a viable but noncultivable biofilm state.
Biofilms with induced dormancy were more likely to survive rifampicin. Furthermore, we found that the reduction of cultivable cells was not sufficient to reach definite conclusions on antimicrobial effectiveness.
本研究旨在使用临床和共生分离株，确定不同休眠细菌比例的表皮葡萄球菌生物膜对抗生素的有效性。
确定表皮葡萄球菌分离株形成休眠状态的能力。通过计数活菌和可培养细胞以及共聚焦显微镜评估预防或诱导休眠的生物膜对抗生素的敏感性。
大多数测试菌株都观察到了休眠。四环素和利福平增强了可培养但不可培养生物膜状态的发展。
诱导休眠的生物膜更有可能耐受利福平。此外，我们发现减少可培养细胞的数量不足以对抗菌效果做出明确的结论。
