Laboratory of Human Molecular Genetics, Universidade Luterana do Brasil (ULBRA), Canoas, Brazil.
Endocrine Division, Porto Alegre, Brazil.
Kidney Blood Press Res. 2017;42(6):1164-1174. doi: 10.1159/000485863. Epub 2017 Dec 8.
BACKGROUND/AIMS: The -1082A>G polymorphism (rs1800896) in the interleukin-10 (IL10) gene has been associated with type 2 diabetes and diabetic retinopathy, but its relationship with diabetic kidney disease (DKD) is uncertain. The aim of this case-control study was to investigate whether the -1082A>G polymorphism is associated with DKD in white Brazilians with type 2 diabetes mellitus.
Genotyping was done by real-time polymerase chain reaction for 597 type 2 diabetic outpatients. The definition of DKD was based on estimated glomerular filtration rate (eGFR) and albuminuria, and the patients were grouped in three categories: no DKD (n=249), mild to moderate DKD (n=222), and severe DKD (n=126).
The frequency of the minor (G) allele in subjects without DKD did not differ from that observed in subjects with DKD (0.35 vs 0.39, respectively; P = 0.192). Genotype frequencies in subjects without DKD were not significantly different from those observed among patients with mild to moderate DKD or severe DKD. However, considering only the eGFR categories as an indicator of renal function, the AG genotype was independently associated with an increased risk of mildly to moderately decreased eGFR (G3a category) and GG genotype was independently associated with increased risk of kidney failure (G5 category) as compared with AA genotype.
Our findings support the hypothesis that the -1082A>G polymorphism in the IL10 gene might be associated with DKD in white Brazilians with type 2 diabetes.
背景/目的：白细胞介素-10（IL10）基因中的-1082A>G 多态性（rs1800896）与 2 型糖尿病和糖尿病视网膜病变有关，但与糖尿病肾病（DKD）的关系尚不确定。本病例对照研究的目的是探讨-1082A>G 多态性是否与白种巴西 2 型糖尿病患者的 DKD 有关。
通过实时聚合酶链反应对 597 名 2 型糖尿病门诊患者进行基因分型。DKD 的定义基于估计的肾小球滤过率（eGFR）和蛋白尿，患者分为三组：无 DKD（n=249）、轻度至中度 DKD（n=222）和重度 DKD（n=126）。
无 DKD 组患者的次要（G）等位基因频率与 DKD 患者无差异（分别为 0.35 和 0.39；P=0.192）。无 DKD 患者的基因型频率与轻度至中度 DKD 或重度 DKD 患者的观察值无显著差异。然而，仅考虑 eGFR 类别作为肾功能的指标，AG 基因型与轻度至中度 eGFR 降低（G3a 类别）的风险增加独立相关，GG 基因型与肾功能衰竭（G5 类别）的风险增加独立相关与 AA 基因型相比。
我们的研究结果支持以下假设，即 IL10 基因中的-1082A>G 多态性可能与白种巴西 2 型糖尿病患者的 DKD 有关。
