OBJECTIVES

To investigate the time-dependent expression of metallothionein （MT） 1A mRNA and MT2A mRNA in contused skeletal muscle of rats.

METHODS

A total of 54 Sprague-Dawley rats were used in this study. The rats were divided into two parts: control group （=6） and contusion groups （0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion, =6）. Total RNA was extracted from skeletal muscle. The expression levels of MT1A mRNA and MT2A mRNA were detected by SYBR Green I real-time PCR.

RESULTS

The expression trends of the two potential marker genes were related to wound age. In addition to 0.5 h, there were significant contrasts between the control group and contused group （<0.05）, about the expression levels of MT1A mRNA and MT2A mRNA in different phases. As the extension of wound age, the relative expression of MT1A mRNA and MT2A mRNA at 1 h, 6 h, 12 h and 18 h after contusion demonstrated upgrade tendency until its expression levels in 18 h peak with 239.41±15.20 and 717.42±50.76, respectively. When time extends to 24 h after injury, the expression of above two marks decreased, respectively. The MT1A mRNA and MT2A mRNA expression levels increased at 30 h and then decreased.

CONCLUSIONS

Determination of MT1A mRNA and MT2A mRNA levels by real-time PCR may be useful for the estimation of wound age.