[MPure-12自动核酸纯化法与Chelex-100法的比较]

[Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method].

作者信息

Shen X, Li M, Wang Y L, Chen Y L, Lin Y, Zhao Z M, Que T Z

机构信息

Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.

Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China.

出版信息

Fa Yi Xue Za Zhi. 2017 Apr;33(2):168-170. doi: 10.3969/j.issn.1004-5619.2017.02.013. Epub 2017 Apr 25.

DOI:10.3969/j.issn.1004-5619.2017.02.013

PMID:29231025

Abstract

OBJECTIVES

To explore the forensic application value of MPure-12 automatic nucleic acid purification （MPure-12 Method） for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents.

METHODS

Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles.

RESULTS

The samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains （20 μL, 15 μL, 10 μL, 5 μL, 1 μL） showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1 μL blood volume by MPure-12 method.

CONCLUSIONS

MPure-12 method is suitable for DNA extraction of a certain concentration blood samples．Chelex-100 method may be better for the extraction of trace blood samples．This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.

摘要

目的

通过从不同DNA含量的血迹及各类生物样本中提取DNA并进行分型，探讨MPure - 12自动核酸纯化法（MPure - 12法）在DNA提取中的法医应用价值。

方法

采集血迹、精液斑、唾液等9种生物样本。采用MPure - 12法和Chelex - 100法提取DNA，随后进行PCR扩增及电泳以获得STR分型图谱。

结果

MPure - 12法成功对毛发根部、口香糖、烟头、肌肉组织、唾液斑、血迹和精液斑等样本进行了分型。唾液样本存在部分等位基因缺失，接触拭子的基因分型结果不理想。此外，所有血迹（20μL、15μL、10μL、5μL、1μL）采用Chelex - 100法均显示出良好的分型结果。但MPure - 12法对1μL血量样本出现等位基因丢失。