[Chelex-100法对滤纸血痕DNA提取影响因素的比较研究]
[Comparative research of the influence factors of DNA extraction of bloodstain on the filter paper with Chelex-100 method].
作者信息
Ba Hua-Jie, Liu Bing-quan, Ma Jun, Zhu Ai-hua, Lin Zi-qing
机构信息
Changzhou Public Security Bureau, Changzhou 213003, China.
出版信息
Fa Yi Xue Za Zhi. 2007 Oct;23(5):347-8.
OBJECTIVE
To improve DNA extraction from bloodstain on the filter paper and to establish a rapid, simple, and cost-effective method for DNA extraction suitable for database construction.
METHODS
Seven hundred and fifty two aged bloodstains on filter paper were randomly divided into four groups. The four different DNA extraction methods were compared with each other, and two DNA extraction methods used for 63 fresh bloodstains on filter paper were also compared with each other.
RESULTS
There were no statistically significant differences observed among the four DNA extraction methods (P > 0.05) for aged bloodstains on filter paper; But the difference between the two DNA extraction methods for fresh bloodstains on filter paper was obviously (P < 0.05).
CONCLUSION
Extraction of DNA samples from aged bloodstains on filter paper can be accomplished by using Chlex-100 methodology directly with no need to wash the bloodstains.
目的
改进从滤纸上血迹中提取DNA的方法，建立一种快速、简便且经济高效的适合数据库建设的DNA提取方法。
方法
将752份滤纸上的陈旧血迹随机分为四组。对四种不同的DNA提取方法进行相互比较，同时也对用于63份滤纸上新鲜血迹的两种DNA提取方法进行相互比较。
结果
对于滤纸上的陈旧血迹，四种DNA提取方法之间未观察到统计学显著差异（P>0.05）；但对于滤纸上新鲜血迹的两种DNA提取方法之间差异明显（P<0.05）。
结论
从滤纸上陈旧血迹中提取DNA样本可直接采用Chelex-100方法，无需清洗血迹。
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